Transcriptome analysis reveals response regulator SO2426-mediated gene expression in Shewanella oneidensis MR-1 under chromate challenge

Journal Article · Tue Jan 01 04:00:00 EST 2008 · BMC Genomics

DOI:https://doi.org/10.1186/1471-2164-9-395· OSTI ID:1081764

Chourey, Karuna ^[1]; Wei, Wei ^[1]; Wan, Xiu-Feng ^[2]; Thompson, Dorothea K. ^[3]

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1. ORNL
2. Centers for Disease Control and Prevention
3. Purdue University

Shewanella oneidensis MR-1 exhibits diverse metal ion-reducing capabilities and thus is of potential utility as a bioremediation agent. Knowledge of the molecular components and regulatory mechanisms dictating cellular responses to heavy metal stress, however, remains incomplete. In a previous work, the S. oneidensis so2426 gene, annotated as a DNA-binding response regulator, was demonstrated to be specifically responsive at both the transcript and protein levels to acute chromate [Cr(VI)] challenge. To delineate the cellular function of SO2426 and its contribution to metal stress response, we integrated genetic and physiological approaches with a genome-wide screen for target gene candidates comprising the SO2426 regulon.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: SC USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1081764
• Journal Information: BMC Genomics, Journal Name: BMC Genomics Journal Issue: 8 Vol. 9; ISSN 1471-2164
• Country of Publication: United States
• Language: English