Methods for detection of methyl-CpG dinucleotides

Dunn, John J.

Methods for detection of methyl-CpG dinucleotides

Patent · Tue Sep 11 04:00:00 EDT 2012

OSTI ID:1078331

Dunn, John J.

The invention provides methods for enriching methyl-CpG sequences from a DNA sample. The method makes use of conversion of cytosine residues to uracil under conditions in which methyl-cytosine residues are preserved. Additional methods of the invention enable to preservation of the context of me-CpG dinucleotides. The invention also provides a recombinant, full length and substantially pure McrA protein (rMcrA) for binding and isolation of DNA fragments containing the sequence 5'-C.sup.MeCpGG-3'. Methods for making and using the rMcrA protein, and derivatives thereof are provided.

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Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC02-98CH10886

Assignee:: Brookhaven Science Associates, LLC (Upton, NY)

Patent Number(s):: 8,263,742

Application Number:: 13/085,219

OSTI ID:: 1078331

Country of Publication:: United States

Language:: English

References (22)

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