An Approach for Assessing the Signature Quality of Various Chemical Assays when Predicting the Culture Media Used to Grow Microorganisms

Technical Report · Thu Jan 31 23:00:00 EST 2013

DOI:https://doi.org/10.2172/1077996· OSTI ID:1077996

Holmes, Aimee E. ^[1]; Sego, Landon H. ^[1]; Webb-Robertson, Bobbie-Jo M. ^[1]; Kreuzer, Helen W. ^[1]; Anderson, Richard M. ^[1]; Unwin, Stephen D. ^[1]; Weimar, Mark R. ^[1]; Tardiff, Mark F. ^[1]; Corley, Courtney D. ^[1]

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1. Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

We demonstrate an approach for assessing the quality of a signature system designed to predict the culture medium used to grow a microorganism. The system was comprised of four chemical assays designed to identify various ingredients that could be used to produce the culture medium. The analytical measurements resulting from any combination of these four assays can be used in a Bayesian network to predict the probabilities that the microorganism was grown using one of eleven culture media. We evaluated combinations of the signature system by removing one or more of the assays from the Bayes network. We measured and compared the quality of the various Bayes nets in terms of fidelity, cost, risk, and utility, a method we refer to as Signature Quality Metrics

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1077996
• Report Number(s): PNNL--22126
• Country of Publication: United States
• Language: English