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Title: Structure of the Integral Membrane Protein CAAX Protease Ste24p

Journal Article · · Science
 [1];  [1];  [2];  [2];  [2];  [3];  [3];  [4];  [1];  [2]
  1. Membrane Protein Structural Biology Consortium (United States); Univ. of Virginia, Charlottesville, VA (United States)
  2. Membrane Protein Structural Biology Consortium (United States); Univ. of Rochester School of Medicine and Dentistry, Rochester, NY (United States)
  3. Membrane Protein Structural Biology Consortium (United States); Hauptman-Woodward Inst., Buffalo, NY (United States)
  4. Membrane Protein Structural Biology Consortium (United States); Hauptman-Woodward Inst., Buffalo, NY (United States); State Univ. of New York, Buffalo, NY (United States)
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Posttranslational lipidation provides critical modulation of the functions of some proteins. Isoprenoids (i.e., farnesyl or geranylgeranyl groups) are attached to cysteine residues in proteins containing C-terminal CAAX sequence motifs (where A is an aliphatic residue and X is any residue). Isoprenylation is followed by cleavage of the AAX amino acid residues and, in some cases, by additional proteolytic cuts. We determined the crystal structure of the CAAX protease Ste24p, a zinc metalloprotease catalyzing two proteolytic steps in the maturation of yeast mating pheromone a -factor. The Ste24p core structure is a ring of seven transmembrane helices enclosing a voluminous cavity containing the active site and substrate-binding groove. The cavity is accessible to the external milieu by means of gaps between splayed transmembrane helices. We hypothesize that cleavage proceeds by means of a processive mechanism of substrate insertion, translocation, and ejection.

Research Organization:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Organization:
National Institutes of Health, New York, NY (United States)
OSTI ID:
1072772
Journal Information:
Science, Vol. 339, Issue 03, 2013; ISSN 0036-8075
Publisher:
AAAS
Country of Publication:
United States
Language:
ENGLISH

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59 BASIC BIOLOGICAL SCIENCES