Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping

Journal Article · Fri Feb 15 00:00:00 EST 2013 · Organic Letters

DOI:https://doi.org/10.1021/ol303328t· OSTI ID:1067959

Yeh, Hsu-Hua; Chang, Shu-Lin; Chiang, Yi-Ming; Bruno, Kenneth S.; Oakley, Berl R.; Wu, Tung-Kung; Wang, Clay C. C.

Heterologous expression of the A. niger NR-PKS gene, e_gw1_19.204 and the adjacent stand-alone R domain gene, est_GWPlus_C_190476 in A. nidulans demonstrated that they belong to a single gene named dtbA. The DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde 1 and 2-ethyl-4,6-dihydroxy-3,5-dimethylbenzaldehyde 2. Generation of DtbA+R-TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1067959
• Report Number(s): PNNL-SA-92219; BM0101020
• Journal Information: Organic Letters, Vol. 15, Issue 4; ISSN 1523-7060
• Publisher: American Chemical Society
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Aspergillus
Polyketide Synthase
Domain Swapping
Thioesterase Domain