Engineering Fungal Nonreducing Polyketide Synthase by Heterologous Expression and Domain Swapping
Heterologous expression of the A. niger NR-PKS gene, e_gw1_19.204 and the adjacent stand-alone R domain gene, est_GWPlus_C_190476 in A. nidulans demonstrated that they belong to a single gene named dtbA. The DtbA protein produces two polyketides, 2,4-dihydroxy-3,5,6-trimethylbenzaldehyde 1 and 2-ethyl-4,6-dihydroxy-3,5-dimethylbenzaldehyde 2. Generation of DtbA+R-TE chimeric PKSs by swapping the DtbA R domain with the AusA (austinol biosynthesis) or ANID_06448 TE domain enabled the production of two metabolites with carboxylic acids replacing the corresponding aldehydes.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1067959
- Report Number(s):
- PNNL-SA-92219; BM0101020
- Journal Information:
- Organic Letters, Journal Name: Organic Letters Journal Issue: 4 Vol. 15; ISSN 1523-7060
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
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