Composition, Reactivity and Regulation of Extracellular Metal-Reducing Structures (Bacterial Nanowires) Produced by Dissimilatory Metal - Reducing Bacteria
Technical Report
·
OSTI ID:1067834
- Univ. of Guelph, ON (Canada); University of Guelph
- Univ. of Guelph, ON (Canada)
This is the final technical report for the project. There were two objectives in the proposal. The first was to describe the composition and function of electrically conductive appendages, known as bacterial nanowires, which resemble pili but are longer and are electrically conductive. They were first identified on the dissimilatory metal-reducing bacteria (DMRB), Shewanella and Geobacter. Specifically, this project investigated the role of these structures in: (i) the reductive transformation of iron oxides as solid phase electron acceptors; (ii) the use of as uranium as a dissolved electron acceptor to form nanocrystalline particles of uraninite upon reduction. The Beveridge group investigated these processes using advanced cryo-transmission electron microscopy (cryoTEM) to visualize the points of connection between the distal ends of nanowires and the effect they have on solid phase Fe minerals. At the same time, immuno-electron microscopy was applied in an attempt to identify where metal reductases and cytochromes are located on the cell surface, or in the nanowires. The second objective was to define the surface physicochemistry of Shewanella spp. in an attempt to decipher how weak bonding (electrostatics and hydrophobicity) affects the adherence of the bacteria to Fe oxides. This bonding could be dictated by the chemistry of lipopolysaccharide (LPS), or the presence/absence of capsular polysaccharide.
- Research Organization:
- Univ. of Guelph, ON (Canada)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- DOE Contract Number:
- FG02-99ER62730
- OSTI ID:
- 1067834
- Report Number(s):
- DOE-ER--62730
- Country of Publication:
- United States
- Language:
- English
Similar Records
Composition, Reactivity, and Regulations of Extracellular Metal-Reducing Structures (Bacterial Nanowires) Produced by Dissimilatory Metal Reducing Bacteria
Composition, Reactivity and Regulation of Extracellular Metal-Reducing Structures (Bacterial Nanowires) Produced by Dissimilatory Metal - Reducing Bacteria
How the xap Locus Put Electrical “Zap” in Geobacter sulfurreducens Biofilms
Technical Report
·
Thu Jun 01 00:00:00 EDT 2006
·
OSTI ID:895881
Composition, Reactivity and Regulation of Extracellular Metal-Reducing Structures (Bacterial Nanowires) Produced by Dissimilatory Metal - Reducing Bacteria
Technical Report
·
Tue Jun 01 00:00:00 EDT 2004
·
OSTI ID:893692
How the xap Locus Put Electrical “Zap” in Geobacter sulfurreducens Biofilms
Journal Article
·
Mon Feb 28 23:00:00 EST 2011
· Journal of Bacteriology
·
OSTI ID:1028543