Organization and Flexibility of Cyanobacterial Thylakoid Membranes Examined by Neutron Scattering

Liberton, Michelle; Page, Lawrence E.; O'Dell, William B.; O'Neill, Hugh; Mamontov, Eugene; Urban, Volker S.; Pakrasi, Himadri B.

doi:10.1074/jbc.m112.416933

Organization and Flexibility of Cyanobacterial Thylakoid Membranes Examined by Neutron Scattering

Journal Article · Fri Feb 01 00:00:00 EST 2013 · Journal of Biological Chemistry

DOI:https://doi.org/10.1074/jbc.m112.416933· OSTI ID:1059289

Liberton, Michelle ^[1]; Page, Lawrence E.; O'Dell, William B.; O'Neill, Hugh; Mamontov, Eugene; Urban, Volker S.; Pakrasi, Himadri B.

Washington Univesity in St. Louis

Cyanobacteria are prokaryotes that can use photosynthesis to convert sunlight into cellular fuel. Knowledge of the organization of the membrane systems in cyanobacteria is critical to understanding the metabolic processes in these organisms. We examined the wild-type strain of Synechocystis sp. PCC 6803 and a series of mutants with altered light-harvesting phycobilisome antenna systems for changes in thylakoid membrane architecture under different conditions. Using small-angle neutron scattering, it was possible to resolve correlation distances of subcellular structures in live cells on the nanometer scale and capture dynamic light-induced changes to these distances. Measurements made from samples with varied scattering contrasts confirmed that these distances could be attributed to the thylakoid lamellar system. We found that the changes to the thylakoid system were reversible between light- and dark-adapted states, demonstrating a robust structural flexibility in the architecture of cyanobacterial cells. Chemical disruption of photosynthetic electron transfer diminished these changes, confirming the involvement of the photosynthetic apparatus. We have correlated these findings with electron microscopy data to understand the origin of the changes in the membranes and found that light induces an expansion in the center-to-center distances between the thylakoid membrane layers. These combined data lend a dynamic dimension to the intracellular organization in cyanobacterial cells.

View Accepted Manuscript (DOE)

Research Organization:: Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC); Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE SC Office of Basic Energy Sciences (SC-22)

Grant/Contract Number:: SC0001035

OSTI ID:: 1059289

Alternate ID(s):: OSTI ID: 1384901
OSTI ID: 1080660

Report Number(s):: DE-SC0001035-65

Journal Information:: Journal of Biological Chemistry, Journal Name: Journal of Biological Chemistry Journal Issue: 5 Vol. 288; ISSN 0021-9258

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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