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Title: Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses

Abstract

A multi-functional liquid chromatography system that performs 1-dimensional, 2-dimensional (strong cation exchange/reverse phase liquid chromatography, or SCX/RPLC) separations, and online phosphopeptides enrichment using a single binary nano-flow pump has been developed. With a simple operation of a function selection valve, which is equipped with a SCX column and a TiO2 (titanium dioxide) column, a fully automated selection of three different experiment modes was achieved. Because the current system uses essentially the same solvent flow paths, the same trap column, and the same separation column for reverse-phase separation of 1D, 2D, and online phosphopeptides enrichment experiments, the elution time information obtained from these experiments is in excellent agreement, which facilitates correlating peptide information from different experiments.

Authors:
; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1059190
Report Number(s):
PNNL-SA-88005
40072; 24698; 400412000
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Journal of Proteome Research, 11(8):4373-4381
Additional Journal Information:
Journal Name: Journal of Proteome Research, 11(8):4373-4381
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Lee, Jung Hwa, Hyung, Seok-Won, Mun, Dong-Gi, Jung, Hee-Jung, Kim, Hokeun, Lee, Hangyeore, Kim, Su-Jin, Park, Kyong Soo, Moore, Ronald J, Smith, Richard D, and Lee, Sang-Won. Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses. United States: N. p., 2012. Web. doi:10.1021/pr3004166.
Lee, Jung Hwa, Hyung, Seok-Won, Mun, Dong-Gi, Jung, Hee-Jung, Kim, Hokeun, Lee, Hangyeore, Kim, Su-Jin, Park, Kyong Soo, Moore, Ronald J, Smith, Richard D, & Lee, Sang-Won. Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses. United States. https://doi.org/10.1021/pr3004166
Lee, Jung Hwa, Hyung, Seok-Won, Mun, Dong-Gi, Jung, Hee-Jung, Kim, Hokeun, Lee, Hangyeore, Kim, Su-Jin, Park, Kyong Soo, Moore, Ronald J, Smith, Richard D, and Lee, Sang-Won. 2012. "Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses". United States. https://doi.org/10.1021/pr3004166.
@article{osti_1059190,
title = {Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses},
author = {Lee, Jung Hwa and Hyung, Seok-Won and Mun, Dong-Gi and Jung, Hee-Jung and Kim, Hokeun and Lee, Hangyeore and Kim, Su-Jin and Park, Kyong Soo and Moore, Ronald J and Smith, Richard D and Lee, Sang-Won},
abstractNote = {A multi-functional liquid chromatography system that performs 1-dimensional, 2-dimensional (strong cation exchange/reverse phase liquid chromatography, or SCX/RPLC) separations, and online phosphopeptides enrichment using a single binary nano-flow pump has been developed. With a simple operation of a function selection valve, which is equipped with a SCX column and a TiO2 (titanium dioxide) column, a fully automated selection of three different experiment modes was achieved. Because the current system uses essentially the same solvent flow paths, the same trap column, and the same separation column for reverse-phase separation of 1D, 2D, and online phosphopeptides enrichment experiments, the elution time information obtained from these experiments is in excellent agreement, which facilitates correlating peptide information from different experiments.},
doi = {10.1021/pr3004166},
url = {https://www.osti.gov/biblio/1059190}, journal = {Journal of Proteome Research, 11(8):4373-4381},
number = ,
volume = ,
place = {United States},
year = {Fri Aug 03 00:00:00 EDT 2012},
month = {Fri Aug 03 00:00:00 EDT 2012}
}