Fully automated multifunctional ultrahigh pressure liquid chromatography system for advanced proteome analyses

Journal Article · Fri Aug 03 00:00:00 EDT 2012 · Journal of Proteome Research, 11(8):4373-4381

DOI:https://doi.org/10.1021/pr3004166· OSTI ID:1059190

Lee, Jung Hwa; Hyung, Seok-Won; Mun, Dong-Gi; Jung, Hee-Jung; Kim, Hokeun; Lee, Hangyeore; Kim, Su-Jin; Park, Kyong Soo; Moore, Ronald J; Smith, Richard D; Lee, Sang-Won

A multi-functional liquid chromatography system that performs 1-dimensional, 2-dimensional (strong cation exchange/reverse phase liquid chromatography, or SCX/RPLC) separations, and online phosphopeptides enrichment using a single binary nano-flow pump has been developed. With a simple operation of a function selection valve, which is equipped with a SCX column and a TiO2 (titanium dioxide) column, a fully automated selection of three different experiment modes was achieved. Because the current system uses essentially the same solvent flow paths, the same trap column, and the same separation column for reverse-phase separation of 1D, 2D, and online phosphopeptides enrichment experiments, the elution time information obtained from these experiments is in excellent agreement, which facilitates correlating peptide information from different experiments.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1059190
• Report Number(s): PNNL-SA-88005; 40072; 24698; 400412000
• Journal Information: Journal of Proteome Research, 11(8):4373-4381, Journal Name: Journal of Proteome Research, 11(8):4373-4381
• Country of Publication: United States
• Language: English

Related Subjects

Environmental Molecular Sciences Laboratory