skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Reproducing Natural Spider Silks' Copolymer Behavior in Synthetic Silk Mimics

Abstract

Dragline silk from orb-weaving spiders is a copolymer of two large proteins, major ampullate spidroin 1 (MaSp1) and 2 (MaSp2). The ratio of these proteins is known to have a large variation across different species of orb-weaving spiders. NMR results from gland material of two different species of spiders, N. clavipes and A. aurantia, indicates that MaSp1 proteins are more easily formed into β-sheet nanostructures, while MaSp2 proteins form random coil and helical structures. To test if this behavior of natural silk proteins could be reproduced by recombinantly produced spider silk mimic protein, recombinant MaSp1/MaSp2 mixed fibers as well as chimeric silk fibers from MaSp1 and MaSp2 sequences in a single protein were produced based on the variable ratio and conserved motifs of MaSp1 and MaSp2 in native silk fiber. Mechanical properties, solid-state NMR, and XRD results of tested synthetic fibers indicate the differing roles of MaSp1 and MaSp2 in the fiber and verify the importance of postspin stretching treatment in helping the fiber to form the proper spatial structure.

Authors:
; ; ; ; ; ;  [1];  [2];  [2];  [2]
  1. Wyoming
  2. (
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
NSFDOE - BASIC ENERGY SCIENCESNIHU.S. AIR FORCE- OFFICE OF SCIENTIFIC RESEARCH
OSTI Identifier:
1057318
Resource Type:
Journal Article
Journal Name:
Biomacromolecules
Additional Journal Information:
Journal Volume: 13; Journal Issue: 12; Journal ID: ISSN 1525-7797
Publisher:
American Chemical Society
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

An, Bo, Jenkins, Janelle E, Sampath, Sujatha, Holland, Gregory P, Hinman, Mike, Yarger, Jeffery L, Lewis, Randolph, Sandia), Utah SU), and AZU). Reproducing Natural Spider Silks' Copolymer Behavior in Synthetic Silk Mimics. United States: N. p., 2012. Web. doi:10.1021/bm301110s.
An, Bo, Jenkins, Janelle E, Sampath, Sujatha, Holland, Gregory P, Hinman, Mike, Yarger, Jeffery L, Lewis, Randolph, Sandia), Utah SU), & AZU). Reproducing Natural Spider Silks' Copolymer Behavior in Synthetic Silk Mimics. United States. doi:10.1021/bm301110s.
An, Bo, Jenkins, Janelle E, Sampath, Sujatha, Holland, Gregory P, Hinman, Mike, Yarger, Jeffery L, Lewis, Randolph, Sandia), Utah SU), and AZU). Tue . "Reproducing Natural Spider Silks' Copolymer Behavior in Synthetic Silk Mimics". United States. doi:10.1021/bm301110s.
@article{osti_1057318,
title = {Reproducing Natural Spider Silks' Copolymer Behavior in Synthetic Silk Mimics},
author = {An, Bo and Jenkins, Janelle E and Sampath, Sujatha and Holland, Gregory P and Hinman, Mike and Yarger, Jeffery L and Lewis, Randolph and Sandia) and Utah SU) and AZU)},
abstractNote = {Dragline silk from orb-weaving spiders is a copolymer of two large proteins, major ampullate spidroin 1 (MaSp1) and 2 (MaSp2). The ratio of these proteins is known to have a large variation across different species of orb-weaving spiders. NMR results from gland material of two different species of spiders, N. clavipes and A. aurantia, indicates that MaSp1 proteins are more easily formed into β-sheet nanostructures, while MaSp2 proteins form random coil and helical structures. To test if this behavior of natural silk proteins could be reproduced by recombinantly produced spider silk mimic protein, recombinant MaSp1/MaSp2 mixed fibers as well as chimeric silk fibers from MaSp1 and MaSp2 sequences in a single protein were produced based on the variable ratio and conserved motifs of MaSp1 and MaSp2 in native silk fiber. Mechanical properties, solid-state NMR, and XRD results of tested synthetic fibers indicate the differing roles of MaSp1 and MaSp2 in the fiber and verify the importance of postspin stretching treatment in helping the fiber to form the proper spatial structure.},
doi = {10.1021/bm301110s},
journal = {Biomacromolecules},
issn = {1525-7797},
number = 12,
volume = 13,
place = {United States},
year = {2012},
month = {10}
}