Genotypic variation in aluminum resistance, cellular aluminum fractions, callose and pectin formation and organic acid accumulation in roots of Populus hybrids

Smith, Ernest; Naik, Dhiraj; Cumming, Jonathan R.

doi:10.1016/j.envexpbot.2011.03.003

Genotypic variation in aluminum resistance, cellular aluminum fractions, callose and pectin formation and organic acid accumulation in roots of Populus hybrids

Journal Article · Thu Sep 01 04:00:00 EDT 2011 · Environmental and Experimental Botany

DOI:https://doi.org/10.1016/j.envexpbot.2011.03.003· OSTI ID:1052827

Smith, Ernest ^[1]; Naik, Dhiraj; Cumming, Jonathan R.

West Virginia University

Soil acidity and aluminum (Al) toxicity are major factors limiting crop yield and forest productivity worldwide. Hybrid poplar (Populus spp.) was used as a model to assess genotypic variation in Al resistance and physiological stress responses to Al in a woody tree species. Eight hybrid crosses of P. trichocarpa, P. deltoides and P. nigra were exposed to Al in solution culture. Resistance to Al varied by genotype and hybrid cross, with P. trichocarpa x P. deltoides crosses being most resistant, P. trichocarpa x P. nigra being intermediate and P. deltoides x P. nigra being most sensitive to Al. Total root Al accumulation was not a good indicator of Al resistance/sensitivity. However, the partitioning of Al into apoplastic and symplastic fractions indicated that differences in sensitivity among genotypes were associated with Al uptake into the symplasm. Aluminum treatment increased callose and pectin concentrations of root tips in all genotypes, but more prominently in Al sensitive genotypes/hybrids. In Al sensitive genotypes, higher levels of symplastic Al accumulation correlated with elevated concentrations of citrate, malate, succinate or formate in root tips, whereas organic acid accumulation was not as pronounced in Al resistant genotypes. These findings suggest that exclusion of Al from the symplast is associated with Al resistance. Further screening of Al tolerant poplar genotypes could yield successful candidates to be utilized for sustainable reforestation/reclamation and carbon sequestration projects where soil acidity may limit tree growth.

Research Organization:: West Virginia Univ., Morgantown, WV (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: FG02-06ER64148

OSTI ID:: 1052827

Report Number(s):: DOE/ER64148-2

Journal Information:: Environmental and Experimental Botany, Journal Name: Environmental and Experimental Botany Journal Issue: 2 Vol. 72; ISSN 0098-8472

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

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54 ENVIRONMENTAL SCIENCES
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Genotypic variation in aluminum resistance, cellular aluminum fractions, callose and pectin formation and organic acid accumulation in roots of Populus hybrids

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References (55)

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