Separation of Variant Methylated Histone Tails by Differential Ion Mobility
Differential ion mobility spectrometry (FAIMS) is emerging as a broadly useful tool for separation of isomeric modified peptides with post-translational modifications (PTMs) attached to alternative residues. Such separations were anticipated to become more challenging for smaller PTMs and longer peptides. Here we show that FAIMS can fully resolve localization variants involving a PTM as minuscule as methylation, even for larger peptides in the middle-down range.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1048610
- Report Number(s):
- PNNL-SA-88466; 40072; 400412000; TRN: US201216%%1043
- Journal Information:
- Analytical Chemistry, Vol. 84, Issue 15
- Country of Publication:
- United States
- Language:
- English
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