Atomic structure of the nuclear pore complex targeting domain of a Nup116 homologue from the yeast, Candida glabrata
The nuclear pore complex (NPC), embedded in the nuclear envelope, is a large, dynamic molecular assembly that facilitates exchange of macromolecules between the nucleus and the cytoplasm. The yeast NPC is an eightfold symmetric annular structure composed of {approx}456 polypeptide chains contributed by {approx}30 distinct proteins termed nucleoporins. Nup116, identified only in fungi, plays a central role in both protein import and mRNA export through the NPC. Nup116 is a modular protein with N-terminal 'FG' repeats containing a Gle2p-binding sequence motif and a NPC targeting domain at its C-terminus. We report the crystal structure of the NPC targeting domain of Candida glabrata Nup116, consisting of residues 882-1034 [CgNup116(882-1034)], at 1.94 {angstrom} resolution. The X-ray structure of CgNup116(882-1034) is consistent with the molecular envelope determined in solution by small-angle X-ray scattering. Structural similarities of CgNup116(882-1034) with homologous domains from Saccharomyces cerevisiae Nup116, S. cerevisiae Nup145N, and human Nup98 are discussed.
- Research Organization:
- Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
- Sponsoring Organization:
- DOE - BASIC ENERGY SCIENCESNIH
- OSTI ID:
- 1047886
- Journal Information:
- Proteins, Journal Name: Proteins Journal Issue: (8) ; 08, 2012 Vol. 80; ISSN PSFGEY; ISSN 0887-3585
- Country of Publication:
- United States
- Language:
- ENGLISH
Similar Records
Structures of the autoproteolytic domain from the Saccharomyces cerevisiae nuclear pore complex component, Nup145
Intra-molecular cohesion of coils mediated by phenylalanine-glycine motifs in the natively unfolded domain of a nucleoporin
Intramolecular Cohesion of Coils Mediated by Phenylalanine–Glycine Motifs in the Natively Unfolded Domain of a Nucleoporin
Journal Article
·
Mon Apr 30 00:00:00 EDT 2012
· Proteins
·
OSTI ID:1037492
Intra-molecular cohesion of coils mediated by phenylalanine-glycine motifs in the natively unfolded domain of a nucleoporin
Journal Article
·
Thu Apr 19 00:00:00 EDT 2007
· PLoS Computational Biology, vol. 4, no. 8, August 8, 2008, e1000145
·
OSTI ID:940865
Intramolecular Cohesion of Coils Mediated by Phenylalanine–Glycine Motifs in the Natively Unfolded Domain of a Nucleoporin
Journal Article
·
Thu Aug 07 20:00:00 EDT 2008
· PLoS Computational Biology (Online)
·
OSTI ID:1627187