Biosensor based on Prussian blue nanocubes/reduced graphene oxide nanocomposite for detection of organophosphorus pesticides

Journal Article · Fri Jul 13 04:00:00 EDT 2012 · Nanoscale

DOI:https://doi.org/10.1039/C2NR30976A· OSTI ID:1047411

Zhang, Lin; Zhang, Aidong; Du, Dan; Lin, Yuehe

We demonstrate a facile procedure to efficiently prepare Prussian blue nanocubes/reduced graphene oxide (PBNCs/rGO) nanocomposite by directly mixing Fe3+ and [Fe(CN)6]3 in the presence of GO in polyethyleneimine aqueous solution, resulting in a novel acetylcholinesterase (AChE) biosensor for detection of organophosphorus pesticides (OPs). The obtained nanocomposite was characterized by X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) microanalysis. It was clearly observed that the nanosheet has been decorated with cubic PB nanoparticles and nearly all the nanoparticles are distributed uniformly only on the surface of the reduced GO. No isolated PB nanoparticles were observed, indicating the strong interaction between PB nanocubes and the reduced GO and the formation of PBNCs/rGO nanocomposite. The obtained PBNCs/rGO based AChE biosensor make the peak potential shift negatively to 220 mV. The AChE biosensor shows rapid response and high sensitivity for detection of monocrotophos. These results suggest that the PBNCs/rGO hybrids nanocomposite exhibited high electrocatalytic activity towards the oxidation of thiocholine, which lead to the sensitive detection of OP pesticides.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1047411
• Report Number(s): PNNL-SA-88931; 400412000
• Journal Information: Nanoscale, Journal Name: Nanoscale Journal Issue: 15 Vol. 4; ISSN 2040-3364
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
AQUEOUS SOLUTIONS
Biosensor
DETECTION
FERROCYANIDES
GRAPHITE
HYBRIDIZATION
MICROANALYSIS
MIXING
OXIDATION
OXIDES
PESTICIDES
POTASSIUM COMPOUNDS
POTENTIALS
SENSITIVITY
STRONG INTERACTIONS
SURFACES
TRANSMISSION ELECTRON MICROSCOPY
X-RAY PHOTOELECTRON SPECTROSCOPY
pesticides