Molecular Architecture of the Yeast Monopolin Complex

Journal Article · Mon Jul 30 04:00:00 EDT 2012 · Cell Reports

DOI:https://doi.org/10.1016/j.celrep.2012.05.012· OSTI ID:1047308

Corbett, Kevin D; Harrison, Stephen C ^[1]

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1. Harvard-Med

The Saccharomyces cerevisiae monopolin complex directs proper chromosome segregation in meiosis I by mediating co-orientation of sister kinetochores on the meiosis I spindle. The monopolin subunits Csm1 and Lrs4 form a V-shaped complex that may directly crosslink sister kinetochores. We report here biochemical characterization of the monopolin complex subunits Mam1 and Hrr25 and of the complete four-protein monopolin complex. By purifying monopolin subcomplexes with different subunit combinations, we have determined the stoichiometry and overall architecture of the full monopolin complex. We have determined the crystal structure of Csm1 bound to a Mam1 fragment, showing how Mam1 wraps around the Csm1 dimer and alters the stoichiometry of kinetochore-protein binding by Csm1. We further show that the kinase activity of Hrr25 is altered by Mam1 binding, and we identify Hrr25 phosphorylation sites on Mam1 that may affect monopolin complex stability and/or kinetochore binding in meiosis.

• Research Organization: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
• Sponsoring Organization: OTHERHHMI
• OSTI ID: 1047308
• Journal Information: Cell Reports, Journal Name: Cell Reports Journal Issue: 6 Vol. 1
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
CHROMOSOMES
CRYSTAL STRUCTURE
DIMERS
MEIOSIS
PHOSPHORYLATION
PHOSPHOTRANSFERASES
SACCHAROMYCES CEREVISIAE
SEGREGATION
STABILITY
STOICHIOMETRY
YEASTS