Mass spectrometry-based proteomics: existing capabilities and future directions

Journal Article · Mon May 21 00:00:00 EDT 2012 · Chemical Society Reviews

DOI:https://doi.org/10.1039/C2CS15331A· OSTI ID:1042525

Angel, Thomas E; Aryal, Uma K; Hengel, Shawna M; Baker, Erin Shammel; Kelly, Ryan T; Robinson, Errol W; Smith, Richard D

Mass spectrometry-based proteomics provides a means for identification, characterization, and quantification of biomolecules that are integral components of the processes essential for life. Characterization of proteins present in a biological system at the proteome and sub-proteomes (e.g., the phosphoproteome, proteoglycome, or degradome/peptidome) levels provides a foundation for understanding fundamental aspects as well as potentially a range of translational applications. Emerging technologies such as ion mobility separations coupled with mass spectrometry and microchip-based - proteome measurements combined with continued enhancement of MS instrumentation and separation techniques, such as reversed phase liquid chromatography and potentially capillary electrophoresis, show great promise for both broad undirected as well as targeted measurements and will be critical for e.g., the proteome-wide characterization of post translational modifications and identification, or the verification, and validation of potential biomarkers of disease. MS-based proteomics is also increasingly demonstrating great potential for contributing to our understanding of the dynamics, reactions, and roles proteins and peptides play advancing our understanding of biology on a system wide level for a wide range of applications, from investigations of microbial communities, bioremediation, and human health and disease states alike.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1042525
• Report Number(s): PNNL-SA-84530; CSRVBR; 40072; 400412000; TRN: US201212%%811
• Journal Information: Chemical Society Reviews, Vol. 41, Issue 10; ISSN 0306-0012
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
BIOLOGY
BIOREMEDIATION
CHROMATOGRAPHY
ELECTROPHORESIS
ION MOBILITY
MASS SPECTROSCOPY
MODIFICATIONS
PEPTIDES
PROTEINS
VALIDATION
VERIFICATION
Proteomics
mass spectrometry
ion mobility
post translation modifications
Environmental Molecular Sciences Laboratory