Flow cytometric analysis of toxicity by vesicating agents in human cells in vitro. (Reannouncement with new availability information)
Some alkylating agents are capable of causing severe blisters (vesication) when they contact human skin. The biochemical basis of vesication is undefined. In this study, we have attempted to establish in vitro models for studying vesication. We have utilized human peripheral blood lymphocytes (PBL) and commercially obtained human epidermal keratinocytes (HEK) with flow cytometry to assess cytotoxicity, DNA change, and biochemical alterations induced by sulfur mustard (HD) and its monofunctional analogue, chloroethyl ethyl sulfide (CEES). Cytotoxic dose-response curves, in the dose range 10 -6 -10 -3 M, have been generated for both alkylating agents using propidium iodide uptake. HD showed a 2-20-fold higher cytotoxic potency than did CEES. HEKs were 10 times more resistant to the cytotoxic effects of both compounds than were human lymphocytes.
- Research Organization:
- Army Medical Research Inst. of Chemical Defense, Aberdeen Proving Ground, MD (United States)
- OSTI ID:
- 104159
- Report Number(s):
- AD-A--243067/6/XAB; USAMRICD-P--89-062
- Country of Publication:
- United States
- Language:
- English
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