Understanding the importance of the viscosity contrast between the sample solvent plug and the mobile phase and its potential consequences in two-dimensional high-performance liquid chromatography

Journal Article · Thu Jan 01 04:00:00 EST 2009 · Journal of Chromatography A

DOI:https://doi.org/10.1016/j.chroma.2008.11.067· OSTI ID:1041467

Shalliker, R. Andrew ^[1]; Guiochon, Georges A ^[2]

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1. University of Western Sydney, Australia
2. ORNL

The effect of solvent viscosity mismatch on elution performance in reversed-phase HPLC was studied using moment analysis. Two conditions were tested: (1) the mobile phase viscosity was less than the injection plug viscosity, and (2) the mobile phase viscosity was greater than the injection plug viscosity. Under the first condition, retention time and elution performance decreased as the viscosity contrast between the mobile phase and injection plug increased. The effect on performance was more marked as the injection volume increased. A decrease in performance of 12% for compounds with retention factors up to 2.8 was apparent even when the viscosity contrast was only 0.165 cP. In the second set of conditions, elution performance was actually observed to increase, by as much as 25% for a 40 {micro}L injection, as the viscosity contrast between the mobile phase and the solute plug increased. No change in the retention factor was observed. This behaviour was attributed to the shape of an injection plug as it enters into the column, whereby a low viscosity plug permeates away from the wall when the column contains a higher viscosity mobile phase, and vice a versa for a high viscosity plug entering a low viscosity mobile phase. At no stage was either a band splitting or shoulders observed with viscosity contrasts up to 1.283 cP, as could have been expected.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1041467
• Journal Information: Journal of Chromatography A, Journal Name: Journal of Chromatography A Journal Issue: 5 Vol. 1216; ISSN 0021-9673; ISSN JOCRAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
PERFORMANCE
RETENTION
SHAPE
SOLUTES
SOLVENTS
VISCOSITY