The ultimate band compression factor in gradient elution chromatography

Journal Article · Tue Jan 01 04:00:00 EST 2008 · Journal of Chromatography A

DOI:https://doi.org/10.1016/j.chroma.2007.11.044· OSTI ID:1041456

Gritti, Fabrice ^[1]; Guiochon, Georges A ^[2]

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1. University of Tennessee, Knoxville (UTK)
2. ORNL

The equations predicting the ultimate time band compression factor, G = (t{sub R}-t{sub F})/t{sub p} in linear gradient elution chromatography, for an infinitely narrow injection (injection time t{sub p} {yields} 0) were derived for an ideal-model column (dispersionless chromatography, H = 0) assuming the Linear Solvent Strength Model for the retention behavior of the analyte. Numerical solutions can readily be obtained when the LSSM model does not apply. The results can be generalized to any retained organic modifier (k'{sub A}) in the mobile phase. The stronger the retention of the organic modifier, the more effective the band compression. Dispersion in real chromatographic column (H {ne} 0) affects the limits that can be reached in linear gradients but poorly in step gradients. Examples based on a conventional HETP of about 12 {micro}m using a 5 {micro}m particle packed column reveal that the best time compression factor that could be expected is twice the one predicted with an ideal column.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1041456
• Journal Information: Journal of Chromatography A, Journal Name: Journal of Chromatography A Journal Issue: 1-2 Vol. 1178; ISSN 0021-9673; ISSN JOCRAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
ACETONITRILE
CAFFEINE
CHROMATOGRAPHY
COMPRESSION
EFFICIENCY
EXTRACTION COLUMNS
ISOTHERMS
NUMERICAL SOLUTION
RETENTION
SIMULATION
SOLVENTS
WATER