Metal-ion dependence of the active-site conformation of the translesion DNA polymerase Dpo4 from Sulfolobus solfataricus
Journal Article
·
· Acta Crystallogr. F
- Vanderbilt
Crystal structures of a binary Mg{sup 2+}-form Dpo4-DNA complex with 1,N{sup 2}-etheno-dG in the template strand as well as of ternary Mg{sup 2+}-form Dpo4-DNA-dCTP/dGTP complexes with 8-oxoG in the template strand have been determined. Comparison of their conformations and active-site geometries with those of the corresponding Ca{sup 2+}-form complexes revealed that the DNA and polymerase undergo subtle changes as a result of the catalytically more active Mg{sup 2+} occupying both the A and B sites.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Organization:
- National Institutes of Health (NIH)
- OSTI ID:
- 1037494
- Journal Information:
- Acta Crystallogr. F, Vol. 66, Issue (9) ; 09, 2010; ISSN 1744-3091
- Country of Publication:
- United States
- Language:
- ENGLISH
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