Thymine DNA glycosylase specifically recognizes 5-carboxylcytosine-modified DNA
- UC
Human thymine DNA glycosylase (hTDG) efficiently excises 5-carboxylcytosine (5caC), a key oxidation product of 5-methylcytosine in genomic DNA, in a recently discovered cytosine demethylation pathway. We present here the crystal structures of the hTDG catalytic domain in complex with duplex DNA containing either 5caC or a fluorinated analog. These structures, together with biochemical and computational analyses, reveal that 5caC is specifically recognized in the active site of hTDG, supporting the role of TDG in mammalian 5-methylcytosine demethylation.
- Research Organization:
- Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
- Sponsoring Organization:
- FOREIGNNIH
- OSTI ID:
- 1037485
- Journal Information:
- Nat. Chem. Biol., Journal Name: Nat. Chem. Biol. Journal Issue: 04, 2012 Vol. 8; ISSN 1552-4450
- Country of Publication:
- United States
- Language:
- ENGLISH
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