Identifying Low pH Active and Lactate-Utilizing Taxa within Oral Microbiome Communities from Healthy Children Using Stable Isotope Probing Techniques

Journal Article · Mon Mar 05 04:00:00 EST 2012 · PLoS One, 7(3):Article No. e32219

DOI:https://doi.org/10.1371/journal.pone.0032219· OSTI ID:1036913

McLean, Jeffrey S; Fansler, Sarah J; Majors, Paul D; Mcateer, Kathleen; Allen, Lisa Z; Shirtliff, Mark E; Lux, Renate; Shi, Wenyuan

Many human microbial infectious diseases including dental caries are polymicrobial in nature and how these complex multi-species communities evolve from a healthy to a diseased state is not well understood. Although many health- or disease-associated oral microbes have been characterized in vitro, their physiology in vivo in the presence of the complex oral microbiome is difficult to determine with current approaches. In addition, about half of these oral species remain uncultivated to date and little is known except their 16S rRNA sequence. Lacking culture-based physiological analyses, the functional roles of uncultivated microorganisms will remain enigmatic despite their apparent disease correlation. To start addressing these knowledge gaps, we applied a novel combination of in vivo Magnetic Resonance Spectroscopy (MRS) with RNA and DNA based Stable Isotope Probing (SIP) to oral plaque communities from healthy children for temporal monitoring of carbohydrate utilization, organic acid production and identification of metabolically active and inactive bacterial species.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1036913
• Report Number(s): PNNL-SA-83915; 25694
• Journal Information: PLoS One, 7(3):Article No. e32219, Journal Name: PLoS One, 7(3):Article No. e32219 Journal Issue: 3 Vol. 7
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
CARBOHYDRATES
CARIES
CHILDREN
COMMUNITIES
DISEASES
DNA
Environmental Molecular Sciences Laboratory
FUNCTIONALS
IN VITRO
IN VIVO
INFECTIOUS DISEASES
MAGNETIC RESONANCE
MICROORGANISMS
MONITORING
ORGANIC ACIDS
PHYSIOLOGY
PRODUCTION
RNA
SPECTROSCOPY
STABLE ISOTOPES
microbiome
oral communities
polymicrobial