Bacterial Growth Phase Influences Methylmercury Production by the Sulfate-Reducing Bacterium Desulfovibrio desulfuricans ND132

Journal Article · Fri Dec 31 23:00:00 EST 2010 · Science of the Total Environment, The

DOI:https://doi.org/10.1016/j.scitotenv.2011.06.037· OSTI ID:1035522

Biswas, Abir ^[1]; Brooks, Scott C ^[1]; Miller, Carrie L ^[1]; Mosher, Jennifer J ^[1]; Yin, Xiangping Lisa ^[1]; Drake, Meghan M ^[1]

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1. ORNL

The effect of bacterial growth phase is an aspect of mercury (Hg) methylation that previous studies have not investigated in detail. Here we consider the effect of growth phase (mid-log, late-log and late stationary phase) on Hg methylation by the known methylator Desulfovibrio desulfuricans ND132. We tested the addition of Hg alone (chloride-complex), Hg with Suwannee River natural organic matter (SRNOM) (unequilibrated), and Hg equilibrated with SRNOM on monomethylmercury (MMHg) production by ND132 over a growth curve in pyruvate fumarate media. This NOM did not affect MMHg production even under very low Hg: SRNOM ratios, where Hg binding is predicted to be dominated by high energy sites. Adding Hg or Hg NOM to growing cultures 24 h before sampling (late addition) resulted in ~2 greater net fraction of Hg methylated than for comparably aged cultures exposed to Hg from the initial culture inoculation (early addition). Mid-and late-log phase cultures produced similar amounts of MMHg, but late stationary phase cultures (both under early and late Hg addition conditions) produced up to ~3 more MMHg, indicating the potential importance of growth phase in studies of MMHg production.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: SC USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1035522
• Journal Information: Science of the Total Environment, The, Journal Name: Science of the Total Environment, The Journal Issue: 19 Vol. 409; ISSN 0048-9697
• Country of Publication: United States
• Language: English