A HPLC method for the quantification of butyramide and acetamide at ppb levels in hydrogeothermal waters
A quantitative analytical method to determine butyramide and acetamide concentrations at the low ppb levels in geothermal waters has been developed. The analytes are concentrated in a preparation step by evaporation and analyzed using HPLC-UV. Chromatographic separation is achieved isocratically with a RP C-18 column using a 30 mM phosphate buffer solution with 5 mM heptane sulfonic acid and methanol (98:2 ratio) as the mobile phase. Absorbance is measured at 200 nm. The limit of detection (LOD) for BA and AA were 2.0 {mu}g L{sup -1} and 2.5 {mu}g L{sup -1}, respectively. The limit of quantification (LOQ) for BA and AA were 5.7 {mu}g L{sup -1} and 7.7 {mu}g L{sup -1}, respectively, at the detection wavelength of 200 nm. Attaining these levels of quantification better allows these amides to be used as thermally reactive tracers in low-temperature hydrogeothermal systems.
- Research Organization:
- Idaho National Laboratory (INL)
- Sponsoring Organization:
- DOE - EE
- DOE Contract Number:
- AC07-05ID14517
- OSTI ID:
- 1035212
- Report Number(s):
- INL/JOU-11-23140
- Journal Information:
- Analytical Methods, Journal Name: Analytical Methods Journal Issue: 2 Vol. 4; ISSN 1759-9660
- Country of Publication:
- United States
- Language:
- English