Improving the diffraction of full-length human selenomethionyl metavinculin crystals by streak-seeding

Journal Article · Thu Jun 28 04:00:00 EDT 2012 · Acta Crystallogr. F

DOI:https://doi.org/10.1107/S1744309110041059· OSTI ID:1033795

Rangarajan, Erumbi S; Izard, Tina ^[1]

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1. Scripps

Metavinculin is an alternatively spliced isoform of vinculin that has a 68-residue insert in its tail domain (1134 total residues) and is exclusively expressed in cardiac and smooth muscle tissue, where it plays important roles in myocyte adhesion complexes. Mutations in the metavinculin-specific insert are associated with dilated cardiomyopathy (DCM) in man. Crystals of a DCM-associated mutant of full-length selenomethionine-labeled metavinculin grown by hanging-drop vapor diffusion diffracted poorly and were highly sensitive to radiation, preventing the collection of a complete X-ray diffraction data set at the highest possible resolution. Streak-seeding markedly improved the stability, crystal-growth rate and diffraction quality of DCM-associated mutant metavinculin crystals, allowing complete data collection to 3.9 {angstrom} resolution. These crystals belonged to space group P4{sub 3}2{sub 1}2, with two molecules in the asymmetric unit and unit-cell parameters a = b = 170, c = 211 {angstrom}, {alpha} = {beta} = {gamma} = 90{sup o}.

• Research Organization: Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
• Sponsoring Organization: INDUSTRYNIH
• OSTI ID: 1033795
• Journal Information: Acta Crystallogr. F, Journal Name: Acta Crystallogr. F Journal Issue: (12) ; 12, 2010 Vol. 66; ISSN 1744-3091
• Country of Publication: United States
• Language: ENGLISH

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ADHESION
CRYSTAL GROWTH
DIFFRACTION
DIFFUSION
MUSCLES
MUTANTS
MUTATIONS
MYOCARDIUM
RESIDUES
RESOLUTION
SPACE GROUPS
STABILITY
X-RAY DIFFRACTION