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Microbial reductive dehalogenation of vinyl chloride

Patent ·
OSTI ID:1032875
 [1];  [2];  [3];  [4];  [5];  [1]
  1. Stanford, CA
  2. Baltimore, MD
  3. Berlin, DE
  4. Nannhein, DE
  5. Los Altos, CA
+ Show Author Affiliations

Compositions and methods are provided that relate to the bioremediation of chlorinated ethenes, particularly the bioremediation of vinyl chloride by Dehalococcoides-like organisms. An isolated strain of bacteria, Dehalococcoides sp. strain VS, that metabolizes vinyl chloride is provided; the genetic sequence of the enzyme responsible for vinyl chloride dehalogenation; methods of assessing the capability of endogenous organisms at an environmental site to metabolize vinyl chloride; and a method of using the strains of the invention for bioremediation.

Research Organization:
Stanford University
Sponsoring Organization:
USDOE Office of Environmental Management (EM)
DOE Contract Number:
FG07-99ER62883
Assignee:
The Board of Trustees of the Leland Stanford Junior University (Palo Alto, CA)
Patent Number(s):
8,063,192
Application Number:
11/659,064
OSTI ID:
1032875
Country of Publication:
United States
Language:
English

References (11)

Reductive Dechlorination of Chlorinated Ethenes and 1,2-Dichloroethane by “Dehalococcoides ethenogenes” 195 journal July 1999
In vitro studies on reductive vinyl chloride dehalogenation by an anaerobic mixed culture. journal January 1997
Growth of a Dehalococcoides-Like Microorganism on Vinyl Chloride and cis-Dichloroethene as Electron Acceptors as Determined by Competitive PCR journal February 2003
Complete Detoxification of Vinyl Chloride by an Anaerobic Enrichment Culture and Identification of the Reductively Dechlorinating Population as a Dehalococcoides Species journal February 2003
Reductive Dechlorination of Tetrachloroethene to Ethene by a Two-Component Enzyme Pathway journal April 1998
Molecular Analysis of Dehalococcoides 16S Ribosomal DNA from Chloroethene-Contaminated Sites throughout North America and Europe journal February 2002
Trichloroethene Reductive Dehalogenase from Dehalococcoides ethenogenes: Sequence of tceA and Substrate Range Characterization journal December 2000
Detoxification of vinyl chloride to ethene coupled to growth of an anaerobic bacterium journal July 2003
Reductive Dechlorination of cis -1,2-Dichloroethene and Vinyl Chloride by “ Dehalococcoides ethenogenes ” journal February 2001
From genes to protein structure and function: novel applications of computational approaches in the genomic era journal January 2000
Isolation of a Bacterium That Reductively Dechlorinates Tetrachloroethene to Ethene journal June 1997

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES