HPTLC Plate Blotting for Liquid Microjunction Surface Sampling Probe Mass Spectrometric Analysis of Analytes Separated on a Wettable Phase Plate

Journal Article · Sun Jan 01 04:00:00 EST 2012 · Rapid Communications in Mass Spectrometry

DOI:https://doi.org/10.1002/rcm.5299· OSTI ID:1032814

Walworth, Matthew J ^[1]; Stankovich, Joseph J ^[1]; Van Berkel, Gary J ^[1]; Schulz, Michael ^[2]; Minarik, susanne ^[2]

---

1. ORNL
2. Merck Research Laboratories

A blotting method that transfers analytes separated on wettable HPTLC plates to a hydrophobic reversed-phase C8 HPLTC plate suitable for analysis with a liquid microjunction surface sampling probe electrospray ionization mass spectrometry system was described and demonstrated. The simple blotting procedure transfers the analyte from the wettable plate to the topmost surface of a rigidly backed, easy-to-mount hydrophobic substrate that already has been proven viable for analysis by this sampling probe/mass spectrometry system. The utility of the approach was demonstrated by the analysis of a four-component peptide mixture originally separated on a ProteoChrom HPTLC cellulose sheet and then blotted to the reversed phase HPTLC plate.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: ORNL work for others; SC USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1032814
• Journal Information: Rapid Communications in Mass Spectrometry, Journal Name: Rapid Communications in Mass Spectrometry Journal Issue: 1 Vol. 26; ISSN RCMSEF; ISSN 0951-4198
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
CELLULOSE
HPTLC
IONIZATION
MASS SPECTROSCOPY
MIXTURES
PEPTIDES
PLATES
PROBES
SAMPLING
SPECTROSCOPY
SUBSTRATES
blotting
electrospray ionization
hydrophobic reversed-phase
liquid microjunction surface sampling probe
mass spectrometry
peptides