Rapid Analysis of Isobaric Exogenous Metabolites by Differential Mobility Spectrometry Mass Spectrometry

Journal Article · Sat Jan 01 04:00:00 EST 2011 · Rapid Communications in Mass Spectrometry

DOI:https://doi.org/10.1002/rcm.5238· OSTI ID:1032804

Parson, Whitney B ^[1]; Schneider, Bradley B ^[2]; Kertesz, Vilmos ^[1]; Corr, Jay ^[3]; Covey, Thomas R. ^[2]; Van Berkel, Gary J ^[1]

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1. ORNL
2. MDS Sciex
3. AB Sciex

The direct separation of isobaric glucuronide metabolites from propranolol dosed tissue extracts by differential mobility spectrometry mass spectrometry (DMS-MS) with the use of a polar gas-phase chemical modifier was demonstrated. The DMS gas-phase separation was able to resolve the isobaric metabolites with separation times on the order of ms instead of mins to hrs typically required when using pre-ionization chromatographic separation methods. Direct separation of isobaric metabolites from the complex tissue extract was validated using standards as well as implementing an HPLC separation prior to the DMS-MS analysis to pre-separate the species of interest. The ability to separate isobaric exogenous metabolites directly from a complex tissue extract is expected to facilitate the drug development process by increasing analytical throughput without the requirement for pre-ionization cleanup or separation strategies.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: ORNL work for others; SC USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1032804
• Journal Information: Rapid Communications in Mass Spectrometry, Journal Name: Rapid Communications in Mass Spectrometry Journal Issue: 22 Vol. 25; ISSN RCMSEF; ISSN 0951-4198
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
MASS SPECTROSCOPY
METABOLITES
SPECTROSCOPY
TISSUE EXTRACTS
differential mobility spectrometry
mass spectrometry
metabolites
pharmaceutical compounds