Correlative fluorescence microscopy and liquid STEM of proteins labeled with quantum dots on intact cells.
- ORNL
Liquid scanning transmission electron microscopy (liquid STEM) was used to image individual quantum dots bound to receptors in COS7 cells. The same regions on intact fixed cells were imaged both with fluorescence microscopy and liquid STEM. The obtained spatial resolution on was 3 nm. The resolution was sufficient to discriminate between quantum dots and gold nanoparticles for double labeling applications.
- Research Organization:
- Oak Ridge National Laboratory (ORNL); Shared Research Equipment Collaborative Research Center
- Sponsoring Organization:
- ORNL work for others
- DOE Contract Number:
- AC05-00OR22725
- OSTI ID:
- 1032795
- Journal Information:
- ACS Nano, Journal Name: ACS Nano Journal Issue: 7 Vol. 4; ISSN 1936-0851
- Country of Publication:
- United States
- Language:
- English
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