Enrichment, isolation and characterization of fungi tolerant to 1-ethyl-3-methylimidazolium acetate
Journal Article
·
· Journal of Applied Microbiology
OSTI ID:1032523
This work aims to characterize microbial tolerance to 1-ethyl-3-methylimidazolium acetate ([C2mim][OAc]), ionic liquid that has emerged as a novel biomass pretreatment for lignocellulosic biomass. Enrichment experiments performed using inocula treated with [C2mim][OAc] under solid and liquid cultivation yielded fungal populationsdominated by Aspergilli. Ionic liquid-tolerant Aspergillus isolates from these enrichments were capable of growing in a radial plate growth assay in the presence of 10% [C2mim][OAc]. When a [C2mim][OAc]-tolerant Aspergillus fumigatus strain was grown in the presence of switchgrass, endoglucanases and xylanases were secreted that retained residual enzymatic activity in the presence of 20% [C2mim][OAc]. The results of the study suggest tolerance to ionic liquids is a general property of Aspergilli. Tolerance to an industrially important ionic liquid was discovered in a fungal genera that is widely used in biotechnology, including biomass deconstruction.
- Research Organization:
- Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (US)
- Sponsoring Organization:
- Earth Sciences Division
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 1032523
- Report Number(s):
- LBNL-4924E
- Journal Information:
- Journal of Applied Microbiology, Journal Name: Journal of Applied Microbiology Journal Issue: 4 Vol. 110; ISSN 1364-5072; ISSN JAMIFK
- Country of Publication:
- United States
- Language:
- English
Similar Records
Community dynamics and glycoside hydrolase activities of thermophilic bacterial consortia adapted to switchgrass
Journal Article
·
Sun May 01 00:00:00 EDT 2011
· Applied and Environmental Microbiology
·
OSTI ID:1048264