A novel nanoparticle-based disposable electrochemical immunosensor for diagnosis of exposure to toxic organophosphorus agents
We present a novel disposable electrochemical immunosensor for highly selective and sensitive detection of organophosphorylated butyrylcholinesterase (OP-BChE), a specific biomarker for exposure to toxic organophosphorus agents. In our new approach, the zirconia nanoparticles (ZrO-2) were employed to selectively capture the OP moiety of OP-BChE adducts, and followed by quantum dot (QD)-tagged anti-BChE antibodies for amplified quantification. The captured CdSe-QD tags can be sensitively detected by stripping voltammetry using in situ bismuth-plating method. The OP agent, diisopropylfluorophosphate (DFP), was selected to prepare OP-BChE adducts in various matrices. The formation of OP-BChE adducts in plasma sample was confirmed using mass spectroscopy. The developed electrochemical immunosensor demonstrates a highly linear voltammetric response over the range of 0.1 to 30 nM OP-BChE. Moreover, the immunosensor has been successfully applied for the detection of OP-BChE adducts in the plasma samples. This novel nanoparticle-based electrochemical immunosensor thus provides an alternative way for designing simple, fast, sensitive, and cost-effective sensing platform for on-site screening/evaluating exposure to a variety of OP agents.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 1029862
- Report Number(s):
- PNNL-SA-82058; 30493; 400412000
- Journal Information:
- Advanced Functional Materials, Journal Name: Advanced Functional Materials Journal Issue: 22 Vol. 21; ISSN 1616-301X
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
77 NANOSCIENCE AND NANOTECHNOLOGY
ADDUCTS
ANTIBODIES
BIOLOGICAL MARKERS
DETECTION
DIAGNOSIS
ELECTROCHEMISTRY
ESTERASES
Environmental Molecular Sciences Laboratory
MASS SPECTROSCOPY
MATRICES
ORGANIC PHOSPHORUS COMPOUNDS
QUANTUM DOTS
TOXICITY
electrochemical immunosensor
nanoparticles