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Title: Structure of bacteriophage [phi]29 head fibers has a supercoiled triple repeating helix-turn-helix motif

Abstract

The tailed bacteriophage {phi}29 capsid is decorated with 55 fibers attached to quasi-3-fold symmetry positions. Each fiber is a homotrimer of gene product 8.5 (gp8.5) and consists of two major structural parts, a pseudohexagonal base and a protruding fibrous portion that is about 110 {angstrom} in length. The crystal structure of the C-terminal fibrous portion (residues 112-280) has been determined to a resolution of 1.6 {angstrom}. The structure is about 150 {angstrom} long and shows three distinct structural domains designated as head, neck, and stem. The stem region is a unique three-stranded helix-turn-helix supercoil that has not previously been described. When fitted into a cryoelectron microscope reconstruction of the virus, the head structure corresponded to a disconnected density at the distal end of the fiber and the neck structure was located in weak density connecting it to the fiber. Thin section studies of Bacillus subtilis cells infected with fibered or fiberless {phi}29 suggest that the fibers might enhance the attachment of the virions onto the host cell wall.

Authors:
;  [1]
  1. (Purdue)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Science Foundation (NSF)
OSTI Identifier:
1028030
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proc. Natl. Acad. Sci. USA; Journal Volume: 108; Journal Issue: (12) ; 03, 2011
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; BACILLUS SUBTILIS; BACTERIOPHAGES; CELL WALL; CRYSTAL STRUCTURE; CRYSTALLOGRAPHY; FIBERS; GENES; MICROSCOPES; RESIDUES; RESOLUTION; SYMMETRY

Citation Formats

Xiang, Ye, and Rossmann, Michael G. Structure of bacteriophage [phi]29 head fibers has a supercoiled triple repeating helix-turn-helix motif. United States: N. p., 2011. Web. doi:10.1073/pnas.1018097108.
Xiang, Ye, & Rossmann, Michael G. Structure of bacteriophage [phi]29 head fibers has a supercoiled triple repeating helix-turn-helix motif. United States. doi:10.1073/pnas.1018097108.
Xiang, Ye, and Rossmann, Michael G. Thu . "Structure of bacteriophage [phi]29 head fibers has a supercoiled triple repeating helix-turn-helix motif". United States. doi:10.1073/pnas.1018097108.
@article{osti_1028030,
title = {Structure of bacteriophage [phi]29 head fibers has a supercoiled triple repeating helix-turn-helix motif},
author = {Xiang, Ye and Rossmann, Michael G.},
abstractNote = {The tailed bacteriophage {phi}29 capsid is decorated with 55 fibers attached to quasi-3-fold symmetry positions. Each fiber is a homotrimer of gene product 8.5 (gp8.5) and consists of two major structural parts, a pseudohexagonal base and a protruding fibrous portion that is about 110 {angstrom} in length. The crystal structure of the C-terminal fibrous portion (residues 112-280) has been determined to a resolution of 1.6 {angstrom}. The structure is about 150 {angstrom} long and shows three distinct structural domains designated as head, neck, and stem. The stem region is a unique three-stranded helix-turn-helix supercoil that has not previously been described. When fitted into a cryoelectron microscope reconstruction of the virus, the head structure corresponded to a disconnected density at the distal end of the fiber and the neck structure was located in weak density connecting it to the fiber. Thin section studies of Bacillus subtilis cells infected with fibered or fiberless {phi}29 suggest that the fibers might enhance the attachment of the virions onto the host cell wall.},
doi = {10.1073/pnas.1018097108},
journal = {Proc. Natl. Acad. Sci. USA},
number = (12) ; 03, 2011,
volume = 108,
place = {United States},
year = {Thu Dec 22 00:00:00 EST 2011},
month = {Thu Dec 22 00:00:00 EST 2011}
}