Simultaneous chromium reduction and phenol degradation in a coculture of Escherichia coli ATCC 33456 and Pseudomonas putida DMP-1

Journal Article · Sat Jul 01 04:00:00 EDT 1995 · Applied and Environmental Microbiology

OSTI ID:102600

Shen, Hai; Wang, Yi-Tin ^[1]

---

1. Univ. of Kentucky, Lexington, KY (United States)

In a defined coculture of a Cr(VI) reducer, Escherichia coli ATCC 33456, and a phenol degrader, Pseudomonas putida DMP-1, simultaneous reduction of Cr(VI) and degradation of phenol was observed. When Cr(VI) was present in the coculture, quantitative transformation of Cr(VI) into Cr(III) proceeded with simultaneous degradation of phenol. Cr(VI) reduction was correlated to phenol degradation in the coculture as demonstrated by a regression analysis of the cumulative Cr(VI) reduction and the cumulative phenol degradation. Both the rate and extent of Cr(VI) reduction and phenol degradation were significantly influenced by the population composition of the coculture. Although Cr(VI) reduction occurred as a result of E. coli metabolism, the rate of phenol degradation by P. putida may become a rate-limiting factor for Cr(VI) reduction at a low population ratio of P. putida to E. coli. Phenol degradation by P. putida was very susceptible to the presence of Cr(VI), whereas Cr(VI) reduction by E. coli was significantly influenced by phenol only when phenol was present at high concentrations (>9 mM). 32 refs., 7 figs., 1 tab.

• OSTI ID: 102600
• Journal Information: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 7 Vol. 61; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CHROMIUM
ESCHERICHIA COLI
PHENOLS
POPULATION DYNAMICS
PSEUDOMONAS
REDUCTION