Structural and Functional Studies Indicate That the EPEC Effector, EspG, Directly Binds p21-Activated Kinase
- Vanderbilt
Bacterial pathogens secrete effectors into their hosts that subvert host defenses and redirect host processes. EspG is a type three secretion effector with a disputed function that is found in enteropathogenic Escherichia coli. Here we show that EspG is structurally similar to VirA, a Shigella virulence factor; EspG has a large, conserved pocket on its surface; EspG binds directly to the amino-terminal inhibitory domain of human p21-activated kinase (PAK); and mutations to conserved residues in the surface pocket disrupt the interaction with PAK.
- Research Organization:
- Advanced Photon Source (APS), Argonne National Laboratory (ANL), Argonne, IL (US)
- Sponsoring Organization:
- OTHERNIH
- OSTI ID:
- 1023669
- Journal Information:
- Biochemistry-US, Journal Name: Biochemistry-US Journal Issue: (6) ; 02, 2011 Vol. 60; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- ENGLISH
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