Myosin II Motors and F-Actin Dynamics Drive the Coordinated Movement of the Centrosome and Soma during CNS Glial-Guided Neuronal Migration

Journal Article · Thu Jan 01 04:00:00 EST 2009 · Neuron

DOI:https://doi.org/10.1016/j.neuron.2009.05.028· OSTI ID:1022022

Solecki, Dr. David ^[1]; Trivedi, Dr. Niraj ^[1]; Govek, Eve-Ellen ^[2]; Kerekes, Ryan A ^[3]; Gleason, Shaun Scott ^[3]; Hatten, Mary E ^[2]

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1. St. Jude Children's Research Hospital
2. Rockefeller University, The
3. ORNL

Lamination of cortical regions of the vertebrate brain depends on glial-guided neuronal migration. The conserved polarity protein Par6{alpha} localizes to the centrosome and coordinates forward movement of the centrosome and soma in migrating neurons. The cytoskeletal components that produce this unique form of cell polarity and their relationship to polarity signaling cascades are unknown. We show that F-actin and Myosin II motors are enriched in the neuronal leading process and that Myosin II activity is necessary for leading process actin dynamics. Inhibition of Myosin II decreased the speed of centrosome and somal movement, whereas Myosin II activation increased coordinated movement. Ectopic expression or silencing of Par6{alpha} inhibited Myosin II motors by decreasing Myosin light-chain phosphorylation. These findings suggest leading-process Myosin II may function to 'pull' the centrosome and soma forward during glial-guided migration by a mechanism involving the conserved polarity protein Par6{alpha}.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: ORNL LDRD Seed-Money
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 1022022
• Journal Information: Neuron, Journal Name: Neuron Journal Issue: 1 Vol. 63; ISSN 0896-6273
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
ACTIN
BRAIN
COORDINATES
DYNAMICS
FUNCTIONS
INHIBITION
MIGRATION
MYOSIN
NERVE CELLS
PHOSPHORYLATION
PROTEINS
VELOCITY
VERTEBRATES