Response to Comments on "A Bacterium That Can Grow Using Arsenic Instead of Phosphorus"

Wolfe-Simon, F; Blum, J S; Kulp, T R; Gordon, G W; Hoeft, S E; Pett-Ridge, J; Stolz, J F; Webb, S M; Weber, P K; Davies, P W; Anbar, A D; Oremland, R S

doi:10.1126/science.1202098

Title: Response to Comments on "A Bacterium That Can Grow Using Arsenic Instead of Phosphorus"

Journal Article · Mon Mar 07 00:00:00 EST 2011 · Science

DOI:https://doi.org/10.1126/science.1202098· OSTI ID:1021547

Wolfe-Simon, F; Blum, J S; Kulp, T R; Gordon, G W; Hoeft, S E; Pett-Ridge, J; Stolz, J F; Webb, S M; Weber, P K; Davies, P W; Anbar, A D; Oremland, R S

Concerns have been raised about our recent study describing a bacterium that can grow using arsenic (As) instead of phosphorus (P). Our data suggested that As could act as a substitute for P in major biomolecules in this organism. Although the issues raised are of investigative interest, we contend that they do not invalidate our conclusions. We argue that while no single line of evidence we presented was sufficient to support our interpretation of the data, taken as an entire dataset we find no plausible alternative to our conclusions. Here we reply to the critiques and provide additional arguments supporting the assessment of the data we reported.

View Journal Article

Cite

Export

Save

Research Organization:: Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-7405-ENG-48

OSTI ID:: 1021547

Report Number(s):: LLNL-JRNL-472592; SCEHDK; TRN: US201117%%150

Journal Information:: Science, Vol. 332, Issue 6034; ISSN 0193-4511

Country of Publication:: United States

Language:: English

References (24)

Coupled Arsenotrophy in a Hot Spring Photosynthetic Biofilm at Mono Lake, California Hoeft, S. E.; Kulp, T. R.; Han, S. Applied and Environmental Microbiology, Vol. 76, Issue 14 https://doi.org/10.1128/AEM.00545-10	journal	May 2010
Comment on "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus" Schoepp-Cothenet, B.; Nitschke, W.; Barge, L. M. Science, Vol. 332, Issue 6034 https://doi.org/10.1126/science.1201438	journal	May 2011
Comment on "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus" Benner, S. A. Science, Vol. 332, Issue 6034 https://doi.org/10.1126/science.1201304	journal	May 2011
Arsenic-75 nuclear magnetic resonance: Study of the interaction of arsenate with various molecules of biological interest Geraldes, C. F. G. C.; Saraiva, M. E.; Dias, B. A. Journal of Inorganic Biochemistry, Vol. 46, Issue 2 https://doi.org/10.1016/0162-0134(92)80013-L	journal	May 1992
Comment on "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus" Cotner, J. B.; Hall, E. K. Science, Vol. 332, Issue 6034 https://doi.org/10.1126/science.1201943	journal	May 2011
Kinetics of the hydrolysis of arsenate(V) triesters Baer, Carl D.; Edwards, John O.; Rieger, Philip H. Inorganic Chemistry, Vol. 20, Issue 3 https://doi.org/10.1021/ic50217a052	journal	March 1981
Kinetics of pyroarsenate hydrolysis in aqueous solution Richmond, Tg; Johnson, Jr; Edwards, Jo Australian Journal of Chemistry, Vol. 30, Issue 6 https://doi.org/10.1071/CH9771187	journal	January 1977
The Protein Data Bank Berman, H. M. Nucleic Acids Research, Vol. 28, Issue 1 https://doi.org/10.1093/nar/28.1.235	journal	January 2000
Comment on "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus" Oehler, S. Science, Vol. 332, Issue 6034 https://doi.org/10.1126/science.1201381	journal	May 2011
Biochemistry of arsenic detoxification Rosen, Barry P. FEBS Letters, Vol. 529, Issue 1 https://doi.org/10.1016/S0014-5793(02)03186-1	journal	August 2002
Are bacteria more like plants or animals? Growth rate and resource dependence of bacterial C : N : P stoichiometry Makino, W.; Cotner, J. B.; Sterner, R. W. Functional Ecology, Vol. 17, Issue 1 https://doi.org/10.1046/j.1365-2435.2003.00712.x	journal	February 2003
Determination of arsenic species: A critical review of methods and applications, 2000–2003 Francesconi, Kevin A.; Kuehnelt, Doris The Analyst, Vol. 129, Issue 5 https://doi.org/10.1039/b401321m	journal	January 2004
Arsenate reductases in prokaryotes and eukaryotes. Mukhopadhyay, Rita; Rosen, Barry P. Environmental Health Perspectives, Vol. 110, Issue suppl 5 https://doi.org/10.1289/ehp.02110s5745	journal	October 2002
Editor's Note Alberts, B. Science, Vol. 332, Issue 6034 https://doi.org/10.1126/science.1208877	journal	May 2011
Arsoniumphospholipid in algae Cooney, R. V.; Mumma, R. O.; Benson, A. A. Proceedings of the National Academy of Sciences, Vol. 75, Issue 9 https://doi.org/10.1073/pnas.75.9.4262	journal	September 1978
A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus Wolfe-Simon, F.; Blum, J. S.; Kulp, T. R. Science, Vol. 332, Issue 6034 https://doi.org/10.1126/science.1197258	journal	December 2010
Comment on "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus" Csabai, I.; Szathmary, E. Science, Vol. 332, Issue 6034 https://doi.org/10.1126/science.1201399	journal	May 2011
Adenosine Triphosphate Content of Marine Bacteria1: atp in Bacteria Hamilton, Robert D.; Holm-Hansen, Osmund Limnology and Oceanography, Vol. 12, Issue 2 https://doi.org/10.4319/lo.1967.12.2.0319	journal	April 1967
XAS Study of Arsenic Coordination in Euglena gracilis Exposed to Arsenite Miot, Jennyfer; Morin, Guillaume; Skouri-Panet, Fériel Environmental Science & Technology, Vol. 42, Issue 14 https://doi.org/10.1021/es703072d	journal	July 2008
Comment on "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus" Borhani, D. W. Science, Vol. 332, Issue 6034 https://doi.org/10.1126/science.1201255	journal	May 2011
Did nature also choose arsenic? Wolfe-Simon, Felisa; Davies, Paul C. W.; Anbar, Ariel D. International Journal of Astrobiology, Vol. 8, Issue 2 https://doi.org/10.1017/S1473550408004394	journal	January 2009
Incorporation of Radioarsenate into Proteins and Nucleic Acids of the Ehrlich Lettré Ascites Carcinoma In Vitro Kay, E. R. M. Nature, Vol. 206, Issue 4982 https://doi.org/10.1038/206371a0	journal	April 1965
Bacterial respiration of arsenic and selenium Stolz, John F.; Oremland, Ronald S. FEMS Microbiology Reviews, Vol. 23, Issue 5 https://doi.org/10.1111/j.1574-6976.1999.tb00416.x	journal	October 1999
Comment on "A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus" Redfield, R. J. Science, Vol. 332, Issue 6034 https://doi.org/10.1126/science.1201482	journal	May 2011

Similar Records

A bacterium that can grow by using arsenic instead of phosphorus

Journal Article · Mon Nov 01 00:00:00 EDT 2010 · Science · OSTI ID:1021547

Wolfe-Simon, F; Blum, J S; Kulp, T R; +9 more

A Bacterium That Can Grow by Using Arsenic Instead of Phosphorus

Journal Article · Tue Nov 30 00:00:00 EST 2010 · Methods in Enzymology, na, na, May 5, 2011, pp. 91-114 · OSTI ID:1021547

Myrold, D D; Pett-Ridge, J; Bottomley, P J

A Bacterium that Can Grow by using Arsenic Instead of Phosphorus

Journal Article · Wed Sep 11 00:00:00 EDT 2013 · Science 332:1163,2011 · OSTI ID:1021547

Wolfe-Simon, F.; Blum, J. Switzer.; Kulp, T. R.; +9 more

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ARSENIC
PHOSPHORUS
BIOCHEMISTRY

Title: Response to Comments on "A Bacterium That Can Grow Using Arsenic Instead of Phosphorus"

Citation Formats

References (24)

Similar Records

Related Subjects