Evaluation of surface sampling method performance for Bacillus Spores on clean and dirty outdoor surfaces.

Technical Report · Wed Jun 01 04:00:00 EDT 2011

DOI:https://doi.org/10.2172/1020519· OSTI ID:1020519

Wilson, Mollye C; Einfeld, Wayne; Boucher, Raymond M; Brown, Gary Stephen; Tezak, Matthew Stephen

Recovery of Bacillus atrophaeous spores from grime-treated and clean surfaces was measured in a controlled chamber study to assess sampling method performance. Outdoor surfaces investigated by wipe and vacuum sampling methods included stainless steel, glass, marble and concrete. Bacillus atrophaeous spores were used as a surrogate for Bacillus anthracis spores in this study designed to assess whether grime-coated surfaces significantly affected surface sampling method performance when compared to clean surfaces. A series of chamber tests were carried out in which known amounts of spores were allowed to gravitationally settle onto both clean and dirty surfaces. Reference coupons were co-located with test coupons in all chamber experiments to provide a quantitative measure of initial surface concentrations of spores on all surfaces, thereby allowing sampling recovery calculations. Results from these tests, carried out under both low and high humidity conditions, show that spore recovery from grime-coated surfaces is the same as or better than spore recovery from clean surfaces. Statistically significant differences between method performance for grime-coated and clean surfaces were observed in only about half of the chamber tests conducted.

• Research Organization: Sandia National Laboratories
• Sponsoring Organization: USDOE
• DOE Contract Number: AC04-94AL85000
• OSTI ID: 1020519
• Report Number(s): SAND2011-4085
• Country of Publication: United States
• Language: English

Related Subjects

36 MATERIALS SCIENCE
BACILLUS
EVALUATION
GLASS
HUMIDITY
MARBLE
OUTDOORS
PERFORMANCE
SAMPLING
SPORES
STAINLESS STEELS