A hydroxycinnamoyltransferase responsible for synthesizing suberin aromatics in Arabidopsis
Suberin, a polyester polymer in the cell wall of terrestrial plants, controls the transport of water and nutrients and protects plant from pathogenic infections and environmental stresses. Structurally, suberin consists of aliphatic and aromatic domains; p-hydroxycinnamates, such as ferulate, p-coumarate, and/or sinapate, are the major phenolic constituents of the latter. By analyzing the 'wall-bound' phenolics of mutant lines of Arabidopsis deficient in a family of acyl-CoA dependent acyltransferase (BAHD) genes, we discovered that the formation of aromatic suberin in Arabidopsis, primarily in seed and root tissues, depends on a member of the BAHD superfamily of enzymes encoded by At5g41040. This enzyme exhibits an {omega}-hydroxyacid hydroxycinnamoyltransferase activity with an in vitro kinetic preference for feruloyl-CoA and 16-hydroxypalmitic acid. Knocking down or knocking out the At5g41040 gene in Arabidopsis reduces specifically the quantity of ferulate in suberin, but does not affect the accumulation of p-coumarate or sinapate. The loss of the suberin phenolic differentially affects the aliphatic monomer loads and alters the permeability and sensitivity of seeds and roots to salt stress. This highlights the importance of suberin aromatics in the polymer's function.
- Research Organization:
- Brookhaven National Lab. (BNL), Upton, NY (United States)
- Sponsoring Organization:
- DOE - OFFICE OF SCIENCE
- DOE Contract Number:
- DE-AC02-98CH10886
- OSTI ID:
- 1019439
- Report Number(s):
- BNL-90627-2010-JA; PNASA6; R&D Project: BO-135; BO-147; KP1102010; TRN: US201115%%114
- Journal Information:
- Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 44; ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
Similar Records
Defective Pollen Wall 2 ( DPW2 ) Encodes an Acyl Transferase Required for Rice Pollen Development
Exploiting members of the BAHD acyltransferase family to synthesize multiple hydroxycinnamate and benzoate conjugates in yeast
Related Subjects
99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE
ARABIDOPSIS
AROMATICS
CELL WALL
ENZYMES
GENES
IN VITRO
KINETICS
MONOMERS
MUTANTS
NUTRIENTS
PERMEABILITY
POLYESTERS
POLYMERS
SEEDS
SENSITIVITY
STRESSES
TRANSPORT
WATER
BAHD superfamily
wall-bound phenolics