skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Host cells and methods for producing 3-methyl-2-buten-1-ol, 3-methyl-3-buten-1-ol, and 3-methyl-butan-1-ol

Patent ·
OSTI ID:1019401
 [1];  [1]
  1. Berkeley, CA
+ Show Author Affiliations

The invention provides for a method for producing a 5-carbon alcohol in a genetically modified host cell. In one embodiment, the method comprises culturing a genetically modified host cell which expresses a first enzyme capable of catalyzing the dephosphorylation of an isopentenyl pyrophosphate (IPP) or dimethylallyl diphosphate (DMAPP), such as a Bacillus subtilis phosphatase (YhfR), under a suitable condition so that 5-carbon alcohol is 3-methyl-2-buten-1-ol and/or 3-methyl-3-buten-1-ol is produced. Optionally, the host cell may further comprise a second enzyme capable of reducing a 3-methyl-2-buten-1-ol to 3-methyl-butan-1-ol, such as a reductase.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC02-05CH11231
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
7,985,567
Application Number:
US Patent Application 12/646,189
OSTI ID:
1019401
Country of Publication:
United States
Language:
English

References (30)

A Study of the Structure–Octane Number Relationship for Hydrocarbons journal July 2004
Reductive biotransformations of organic compounds by cells or enzymes of yeast journal July 1990
Structures of Phosphate and Trivanadate Complexes of Bacillus stearothermophilus Phosphatase PhoE: Structural and Functional Analysis in the Cofactor-dependent Phosphoglycerate Mutase Superfamily journal January 2003
Genome-wide Analysis of Substrate Specificities of the Escherichia coli Haloacid Dehalogenase-like Phosphatase Family journal September 2006
A Soluble Form of Phosphatase in Saccharomyces cerevisiae Capable of Converting Farnesyl Diphosphate Into E-E Farnesol journal January 2006
The Nudix hydrolase superfamily journal December 2005
Biotransformation of monoterpene alcohols bySaccharomyces cerevisiae,Torulaspora delbrueckii andKluyveromyces lactis journal April 2000
A cofactor-dependent phosphoglycerate mutase homolog from Bacillus stearothermophilus is actually a broad specificity phosphatase journal September 2001
Toxicity Caused by Hydroxycinnamoyl-Coenzyme A Thioester Accumulation in Mutants of Acinetobacter sp. Strain ADP1 journal May 2004
Overproduction of a Functional Fatty Acid Biosynthetic Enzyme Blocks Fatty Acid Synthesis inEscherichia coli journal September 1998
Identification, Evolution, and Essentiality of the Mevalonate Pathway for Isopentenyl Diphosphate Biosynthesis in Gram-Positive Cocci journal August 2000
The activity of yeast ADH I and ADH II with long-chain alcohols and diols journal January 2001
Complete genome sequence of the alkaliphilic bacterium Bacillus halodurans and genomic sequence comparison with Bacillus subtilis journal November 2000
Overexpression of a cytosolic hydroxymethylglutaryl-CoA reductase leads to squalene accumulation in yeast journal January 1998
Phosphoryl group transfer: evolution of a catalytic scaffold journal September 2004
Metabolic Engineering to Produce Sesquiterpenes in Yeast journal May 2003
Production of the antimalarial drug precursor artemisinic acid in engineered yeast journal April 2006
High-Level Production of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Fed-Batch Culture of Recombinant Escherichia coli journal October 1999
Structures and mechanisms of Nudix hydrolases journal January 2005
Metabolic engineering of Escherichia coli for improved 6-deoxyerythronolide B production journal August 2003
Mono and diterpene production in Escherichia coli journal January 2004
Cloning of a Gene Cluster Encoding Enzymes Responsible for the Mevalonate Pathway from a Terpenoid-antibiotic-producing Streptomyces Strain journal January 2001
Highly Stereoselective Reagents for β-Keto Ester Reductions by Genetic Engineering of Baker's Yeast journal February 2001
‘New uses for an Old Enzyme’ – the Old Yellow Enzyme family of flavoenzymes journal June 2002
Identification of Isopentenol Biosynthetic Genes from Bacillus subtilis by a Screening Method Based on Isoprenoid Precursor Toxicity journal August 2007
On the mechanism of action of the antifungal agent propionate: Propionyl-CoA inhibits glucose metabolism in Aspergillus nidulans journal July 2004
Heterologous Mevalonate Production in Streptomyces lividans TK23 journal January 2004
Analysis of the Function of a Putative 2,3-Diphosphoglyceric Acid-Dependent Phosphoglycerate Mutase fromBacillus subtilis journal July 2000
Characterization of a Saccharomyces cerevisiae NADP(H)-dependent alcohol dehydrogenase (ADHVII), a member of the cinnamyl alcohol dehydrogenase family: Yeast NADP(H)-dependent alcohol dehydrogenase journal October 2002
Engineering a mevalonate pathway in Escherichia coli for production of terpenoids journal June 2003

Similar Records

Related Subjects

60 APPLIED LIFE SCIENCES