Analysis of electrospray derived ions using a quadrupole ion trap mass spectrometer

Conference · Thu Oct 01 04:00:00 EDT 1992

OSTI ID:10180032

Van Berkel, G J

Electrospray (ES) ionization has emerged as a simple and sensitive method by which to produce gas-phase ions from involatile, polar, and thermally labile compounds for analysis by mass spectrometry (MS). Much of the recent interest in ES stems from the ability to produce gas-phase multiply charged ions from high mass biopolymers such as proteins and oligonucleotides. This multiple charging phenomenon effectively extends the mass range of the mass spectrometer and allows for replicate measurements of the molecular weigh thereby increasing the accuracy of the measurement. Moreover, because analytes are sprayed from solution at atmospheric pressure, ES provides a convenient means by which to couple MS and any one of several condensed-phase separation techniques. Another significant advance in MS over the last several years has been the development of the quadrupole ion trap as an analytical mass spectrometer. The ion trap is a small, rugged and relatively low cost mass spectrometer that offers high sensitivity (low detection limits) and the ability to perform high efficiency MS/MS and MS{reg_sign} experiments. These attributes along with the ion trapping nature of the instrument and the more recently demonstrated extended mass-to-charge range and high mass resolution capability make this instrument extremely versatile and well suited as the analyzer for ES derived ions.

• Research Organization: Oak Ridge National Lab., TN (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States); Department of Health and Human Services, Washington, DC (United States)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 10180032
• Report Number(s): CONF-9209208--1; ON: DE93000979; CNN: Grant R01GM45372
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102
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59 BASIC BIOLOGICAL SCIENCES
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HEMOGLOBIN
MASS SPECTROMETERS
QUADRUPOLES
SENSITIVITY