Molecular biology of coal bio-desulfurization. Quarterly technical progress report, April 1--June 30, 1992

Technical Report · Mon Jul 20 04:00:00 EDT 1992

OSTI ID:10176568

Young, K D; Gallagher, J R

Desulfurization genes from Rhodococcus rhodochrous IGTS8 have been subcloned and sequencing began. A 9.0 kb EcoRI DNA fragment, the smallest so far identified that can transfer the desulfurization phenotype from IGTS8 to other organisms, has been sequenced and more than 13 potential open reading frames have been observed. This result implies that the number of genes and protein products involved in desulfurization may be larger than previously expected. To date, none of these open reading frames match any known proteins in the Swiss-Prot database, indicating that the cloned IGTS8 genes are unique. Mutations have been constructed of genes cloned from the pseudomanas isolate, C18 which can clarify the gene products required for the degradation of DBT. The products of two genes, doxH and doxJ, can apparently substitute for one another, but the products of two other genes, doxE and doxF, cannot do so. Progress has been slowed in screening for the desulfurization genes from Desulfovibrio desulfuricans.

• Research Organization: North Dakota Univ., Grand Forks, ND (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: AC22-89PC89901
• OSTI ID: 10176568
• Report Number(s): DOE/PC/89901--T15; ON: DE92040452
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
010402
550200
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
BIOLOGICAL PATHWAYS
COAL
DESULFOVIBRIO
DESULFURIZATION
DNA SEQUENCING
DNA-CLONING
GENE MUTATIONS
GENETIC MAPPING
LYASES
MOLECULAR BIOLOGY
PROGRESS REPORT
PURIFICATION AND UPGRADING
SULFATE-REDUCING BACTERIA