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Title: Thermal and acid tolerant beta-xylosidases, genes encoding, related organisms, and methods

Patent ·
OSTI ID:1016744

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof are provided. Further provided are methods of at least partially degrading xylotriose and/or xylobiose using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof.

Research Organization:
Idaho National Laboratory (INL), Idaho Falls, ID (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC07-99ID13727
Assignee:
Battelle Energy Alliance, LLC (Idaho Falls, ID)
Patent Number(s):
7,923,234
Application Number:
US Patent Application 12/321,636
OSTI ID:
1016744
Country of Publication:
United States
Language:
English

References (45)

Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability journal March 2001
Polymerase chain reaction journal November 1989
Thermoacidophilic Microorganisms and their Novel Biocatalysts journal December 2004
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine journal September 1999
Glycosylation Engineering: Controlling personalities tame wild sugars on proteins and natural products journal September 2006
Recent advances in heterologous gene expression in Escherichia coli journal October 1993
Prediction of protein function from protein sequence and structure journal January 1999
X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins journal January 2004
Xylanases, xylanase families and extremophilic xylanases journal January 2005
Purification and Properties of Acid Stable Xylanases from Aspergillus kawachii journal January 1992
Improved tools for biological sequence comparison. journal April 1988
Temple, W. J., et al. Childhood melanoma journal January 1992
Isolation and characterization of a new family 42 β-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: Identification of the active site residues journal February 2008
Maltose and Maltodextrin Transport in the Thermoacidophilic Gram-Positive Bacterium Alicyclobacillus acidocaldarius Is Mediated by a High-Affinity Transport System That Includes a Maltose Binding Protein Tolerant to Low pH journal November 2000
Strand displacement amplification—an isothermal, in vitro DNA amplification technique journal January 1992
Microbial Cellulose Utilization: Fundamentals and Biotechnology journal September 2002
Ethanol from lignocellulosic biomass techno-economic performance in short-, middle- and long-term journal April 2005
Baculovirus systems for the expression of human gene products journal October 1993
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different journal April 2001
Immunogenicity and protective efficacy of a tuberculosis DNA vaccine journal August 1996
Continuous cultures of fused cells secreting antibody of predefined specificity journal August 1975
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids journal November 1998
Manufacture of Xylose-Based Fermentation Media from Corncobs by Posthydrolysis of Autohydrolysis Liquors journal January 2001
Rapid chemiluminescent nucleic acid assays for detection of TEM-1 beta-lactamase-mediated penicillin resistance in Neisseria gonorrhoeae and other bacteria. journal January 1988
The Effect of Flow Rate of Compressed Hot Water on Xylan, Lignin, and Total Mass Removal from Corn Stover journal September 2003
A thermoacidophilic endoglucanase (CelB) from Alicyclobacillus acidocaldarius displays high sequence similarity to arabinofuranosidases belonging to family 51 of glycoside hydrolases journal August 2003
Yeast systems for the expression of heterologous gene products journal October 1993
Biochemistry and genetics of microbial xylanases journal June 1996
Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format. journal February 1989
Analytical strategies for the use of DNA probes journal February 1988
The acid hydrolysis of sugarcane bagasse hemicellulose to produce xylose, arabinose, glucose and other products journal November 2002
Single molecule detection of RNA reporter probes by amplification with Qβ replicase journal August 1996
Functional reconstitution of a maltose ATP-binding cassette transporter from the thermoacidophilic gram-positive bacterium Alicyclobacillus acidocaldarius journal May 2004
Bacterial glycoproteins: Functions, biosynthesis and applications journal April 2003
Synthesis of an amplifiable reporter RNA for bioassays journal January 1986
Microbial hemicellulases journal June 2003
Purification, Properties and Structural Aspects of a Thermoacidophilic alpha-Amylase from Alicyclobacillus Acidocaldarius Atcc 27009 journal December 1994
Gene cloning, sequencing, and characterization of a family 9 endoglucanase (CelA) with an unusual pattern of activity from the thermoacidophile Alicyclobacillus acidocaldarius ATCC27009 journal December 2002
A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes journal January 1988
Cloning and characterization of two glycosidases from the acidothermophile Alicyclobacillus acidocaldarius ATCC27009 text January 2004
The Synthesis of Bovine Insulin by the Solid Phase Method journal November 1966
Current applications of COS cell based transient expression systems journal October 1993
Cloning and transcriptional analysis of the Thermoanaerobacter ethanolicus strain 39E maltose ABC transport system journal March 2002
Autocatalytic replication of a recombinant RNA journal December 1983
Lignocellulose biodegradation: Fundamentals and applications journal June 2002