[Conversion of acetic acid to methane by thermophiles]. Annual progress report

Technical Report · Tue Feb 01 04:00:00 EST 1994

DOI:https://doi.org/10.2172/10158582· OSTI ID:10158582

Zinder, S H

Acetate is the precursor of approximately two-thirds of the methane produced by anaerobic bioreactors and many other methanogenic habitats. Besides their intrinsic interest, thermophilic acetotrophic methanogenic cultures usually grow at least twice as fast as their mesophilic counterparts, making them more amenable to study. In recent years, attention has been mainly focused on the thermophilic acetate utilizing methanogen Methanothrix strain CALS-1. Methanothrix, also called Methanosaeta, is one of only two methanogenic genera known to convert acetate to methane, the other being Methanosarcina. The faster-growing more versatile Methanosarcina has been better studied. However, when one examines anaerobic digestor contents, Methanothrix is often the dominant acetate-utilizing methanogen. As described in previous progress reports, the authors have achieved methanogenesis from acetate in cell-free extracts of Methanothrix strain CALS-1 grown in a pH auxostat. Using these cell extracts, specific activities for methanogenesis from acetate and ATP of 100--300 nmol/min were routinely obtained, levels comparable to the rate in whole cells, which is not usually the case in methanogenic extracts. Recently obtained results are given and discussed for the following: Methanogenesis in crude extracts; Role of the cell membrane in methanogenesis from acetate; Carbon monoxide dehydrogenase; Novel thermophilic cultures converting acetate to methane; and Methanol-utilizing methanogen.

• Research Organization: Cornell Univ., Ithaca, NY (United States)
• Sponsoring Organization: USDOE, Washington, DC (United States)
• DOE Contract Number: FG02-85ER13370
• OSTI ID: 10158582
• Report Number(s): DOE/ER/13370--T3; ON: DE94012478; BR: KC0600000
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090900
560300
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACETIC ACID
ANAEROBIC DIGESTION
ATP-ASE
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CHEMICALS METABOLISM AND TOXICOLOGY
EXPERIMENTAL DATA
METHANE
METHANOGENIC BACTERIA
OXIDOREDUCTASES
PERFORMANCE TESTING
PROCESSING
PROGRESS REPORT
THERMOPHILIC CONDITIONS