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Title: Solid phase sequencing of biopolymers

Abstract

This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.

Inventors:
 [1];  [2]
  1. Del Mar, CA
  2. La Jolla, CA
Publication Date:
Research Org.:
Sequenom, Inc. (San Diego, CA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1014563
Patent Number(s):
7,803,529
Application Number:
US Patent Application 09/395,409
Assignee:
Sequenom, Inc. (San Diego, CA)
DOE Contract Number:  
FG02-93ER61609
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Cantor, Charles, and Koster, Hubert. Solid phase sequencing of biopolymers. United States: N. p., 2010. Web.
Cantor, Charles, & Koster, Hubert. Solid phase sequencing of biopolymers. United States.
Cantor, Charles, and Koster, Hubert. Tue . "Solid phase sequencing of biopolymers". United States. https://www.osti.gov/servlets/purl/1014563.
@article{osti_1014563,
title = {Solid phase sequencing of biopolymers},
author = {Cantor, Charles and Koster, Hubert},
abstractNote = {This invention relates to methods for detecting and sequencing target nucleic acid sequences, to mass modified nucleic acid probes and arrays of probes useful in these methods, and to kits and systems which contain these probes. Useful methods involve hybridizing the nucleic acids or nucleic acids which represent complementary or homologous sequences of the target to an array of nucleic acid probes. These probes comprise a single-stranded portion, an optional double-stranded portion and a variable sequence within the single-stranded portion. The molecular weights of the hybridized nucleic acids of the set can be determined by mass spectroscopy, and the sequence of the target determined from the molecular weights of the fragments. Nucleic acids whose sequences can be determined include DNA or RNA in biological samples such as patient biopsies and environmental samples. Probes may be fixed to a solid support such as a hybridization chip to facilitate automated molecular weight analysis and identification of the target sequence.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {9}
}

Patent:

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