Imaging separation of neuronal from vascular effects of cocaine on rat cortical brain in vivo
MRI techniques to study brain function assume coupling between neuronal activity, metabolism and flow. However, recent evidence of physiological uncoupling between neuronal and cerebrovascular events highlights the need for methods to simultaneously measure these three properties. We report a multimodality optical approach that integrates dual-wavelength laser speckle imaging (measures changes in blood flow, blood volume and hemoglobin oxygenation), digital-frequency-ramping optical coherence tomography (images quantitative 3D vascular network) and Rhod2 fluorescence (images intracellular calcium for measure of neuronal activity) at high spatiotemporal resolutions (30 {micro}m, 10 Hz) and over a large field of view (3 x 5 mm{sup 2}). We apply it to assess cocaine's effects in rat cortical brain and show an immediate decrease 3.5 {+-} 0.9 min, phase (1) in the oxygen content of hemoglobin and the cerebral blood flow followed by an overshoot 7.1 {+-} 0.2 min, phase (2) lasting over 20 min whereas Ca{sup 2+} increased immediately (peaked at t = 4.1 {+-} 0.4 min) and remained elevated. This enabled us to identify a delay (2.9 {+-} 0.5 min) between peak neuronal and vascular responses in phase 2. The ability of this multimodality optical approach for simultaneous imaging at high spatiotemporal resolutions permits us to distinguish the vascular versus cellular changes of the brain, thus complimenting other neuroimaging modalities for brain functional studies (e. g., PET, fMRI).
- Research Organization:
- BROOKHAVEN NATIONAL LABORATORY (BNL)
- Sponsoring Organization:
- DOE - OFFICE OF SCIENCE
- DOE Contract Number:
- AC02-98CH10886
- OSTI ID:
- 1014362
- Report Number(s):
- BNL--95061-2011-JA; KP1602010
- Journal Information:
- NeuroImage, Journal Name: NeuroImage Journal Issue: 2 Vol. 54
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BLOOD
BLOOD FLOW
BRAIN
CALCIUM
COCAINE
FLUORESCENCE
FUNCTIONALS
HEMOGLOBIN
IN VIVO
LASERS
METABOLISM
OXYGEN
PET (positron emission tomography)
TOMOGRAPHY
calcium (Ca)
cerebral blood flow
cocaine
drug abuse and addiction
fMRI (funtional magnetic resonance imaging)
optical imaging