Protein- protein interaction detection system using fluorescent protein microdomains

Patent · 23 February 2010

OSTI ID:1014181

Waldo, Geoffrey S ^[1]; Cabantous, Stephanie ^[2]

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1. Santa Fe, NM
2. Los Alamos, NM

The invention provides a protein labeling and interaction detection system based on engineered fragments of fluorescent and chromophoric proteins that require fused interacting polypeptides to drive the association of the fragments, and further are soluble and stable, and do not change the solubility of polypeptides to which they are fused. In one embodiment, a test protein X is fused to a sixteen amino acid fragment of GFP (.beta.-strand 10, amino acids 198-214), engineered to not perturb fusion protein solubility. A second test protein Y is fused to a sixteen amino acid fragment of GFP (.beta.-strand 11, amino acids 215-230), engineered to not perturb fusion protein solubility. When X and Y interact, they bring the GFP strands into proximity, and are detected by complementation with a third GFP fragment consisting of GFP amino acids 1-198 (strands 1-9). When GFP strands 10 and 11 are held together by interaction of protein X and Y, they spontaneous association with GFP strands 1-9, resulting in structural complementation, folding, and concomitant GFP fluorescence.

• Research Organization: Los Alamos National Security, LLC (Los Alamos, NM)
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-36
• Assignee: Los Alamos National Security, LLC (Los Alamos, NM)
• Patent Number(s): 7,666,606
• Application Number: US Patent Application 11/295,368
• OSTI ID: 1014181
• Country of Publication: United States
• Language: English