skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid

Abstract

A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reaction chamber is amplified. The magnetic beads and the nucleic acid are separated into a waste stream containing the magnetic beads and a post polymerase chain reaction mix containing the nucleic acid. The reaction mix containing the nucleic acid flows to an analysis unit in the channel for analysis.

Inventors:
 [1]
  1. Livermore, CA
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1013411
Patent Number(s):
7,867,713
Application Number:
12/106,390
Assignee:
LLNL
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Nasarabadi, Shanavaz. Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid. United States: N. p., 2011. Web.
Nasarabadi, Shanavaz. Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid. United States.
Nasarabadi, Shanavaz. Tue . "Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid". United States. https://www.osti.gov/servlets/purl/1013411.
@article{osti_1013411,
title = {Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid},
author = {Nasarabadi, Shanavaz},
abstractNote = {A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reaction chamber is amplified. The magnetic beads and the nucleic acid are separated into a waste stream containing the magnetic beads and a post polymerase chain reaction mix containing the nucleic acid. The reaction mix containing the nucleic acid flows to an analysis unit in the channel for analysis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 11 00:00:00 EST 2011},
month = {Tue Jan 11 00:00:00 EST 2011}
}

Patent:

Save / Share: