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Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid

Patent ·
OSTI ID:1013411
 [1]
  1. Livermore, CA
+ Show Author Affiliations
A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reaction chamber is amplified. The magnetic beads and the nucleic acid are separated into a waste stream containing the magnetic beads and a post polymerase chain reaction mix containing the nucleic acid. The reaction mix containing the nucleic acid flows to an analysis unit in the channel for analysis.
Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC52-07NA27344
Assignee:
LLNL
Patent Number(s):
7,867,713
Application Number:
12/106,390
OSTI ID:
1013411
Country of Publication:
United States
Language:
English

References (5)

PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets journal September 2005
Development of an integrated automation system with a magnetic bead-mediated nucleic acid purification device for genetic analysis and gene manipulation journal January 2004
Automation of laboratory testing for infectious diseases using the polymerase chain reaction — our past, our present, our future journal January 2001
A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents journal April 2005
Fabrication and characterization of polymer microfluidic devices for bio-agent detection conference January 2005

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