Polynucleotides encoding anti-sulfotyrosine antibodies

Patent · Tue Jan 11 04:00:00 EST 2011

OSTI ID:1013407

Bertozzi, Carolyn R ^[1]; Kehoe, John ^[2]; Bradbury, Andrew M ^[3]

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1. Berkeley, CA
2. Saint Davids, PA
3. Santa Fe, NM

The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.

• Research Organization: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-36
• Assignee: Los Alamos National Security, LLC (Los Alamos, NM)
• Patent Number(s): 7,868,152
• Application Number: 12/560,294
• OSTI ID: 1013407
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES