Nuclear magnetic resonance studies of the regulation of the pentose phosphate pathway

Thesis/Dissertation · Fri Nov 01 00:00:00 EST 1991

DOI:https://doi.org/10.2172/10133997· OSTI ID:10133997

Bolo, Nicolas Robin ^[1]

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1. Univ. of California, Berkeley, CA (United States)

The goal of this work is to investigate the potential for and limitations of in vivo nuclear magnetic resonance (NMR) spectroscopy for quantitation of glucose flux through the pentose phosphate pathway (shunt). Interest in the shunt is motivated by the possibility that its activity may be greatly increased in cancer and in the pathological states of cardiac and cerebral ischemia. The ability to dynamically monitor flux through the pentose shunt can give new knowledge about metabolism in pathological states. ¹³C NMR spectroscopy was used to monitor shunt activity by determination of the ratios of [¹³C-4] to [¹³C-5]-glutamate, [¹³C-3] to [¹³C-2]-alanine or [¹³C-3] to [¹³C-2]-lactate produced when [¹³C-2]-glucose is infused. These methods provide measures of the effect of oxidative stresses on shunt activity in systems ranging from cell free enzyme-substrate preparations to cell suspensions and whole animals. In anaerobic cell free preparations, the fraction of glucose flux through the shunt was monitored with a time resolution of 3 minutes. This work predicts the potential for in vivo human studies of pentose phosphate pathway activity based on the mathematical simulation of the ¹³C fractional enrichments of C4 and C5-glutamate as a function of shunt activity and on the signal-to- noise ratio acquired in ¹³C NMR human studies from the current literature.

• Research Organization: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE Office of Science (SC)
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 10133997
• Report Number(s): LBL-31592; ON: DE92010257; CNN: Grant HL07367
• Resource Relation: Other Information: TH: Thesis (Ph.D.); PBD: Nov 1991
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
75 CONDENSED MATTER PHYSICS
SUPERCONDUCTIVITY AND SUPERFLUIDITY
GLUCOSE
METABOLISM
BRAIN
BIOCHEMISTRY
PENTOSES
NUCLEAR MAGNETIC RESONANCE
IN VIVO
CARBON 13
ALANINES
LACTATES
AEROBIC CONDITIONS
NEOPLASMS
RATS
YEASTS
550500
550200
665100
NUCLEAR TECHNIQUES IN CONDENSED MATTER PHYSICS