DNA sequencing by a single molecule detection of labeled nucleotides sequentially cleaved from a single strand of DNA
- Los Alamos National Lab., NM (United States)
- Life Technologies, Inc., Gaithersburg, MD (United States)
We are developing a laser-based technique for the rapid sequencing of large DNA fragments (several kb in size) at a rate of 100 to 1000 bases per second. Our approach relies on fluorescent labeling of the bases in a single fragment of DNA, attachment of this labeled DNA fragment to a support, movement of the supported DNA into a flowing sample stream, sequential cleavage of the end nucleotide from the DNA fragment with an exonuclease, and detection of the individual fluorescently labeled bases by laser-induced fluorescence.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Organization:
- USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 10131004
- Report Number(s):
- LA-UR-93-362; CONF-930159-8; ON: DE93007331
- Resource Relation:
- Journal Volume: 1891; Conference: OE/LASE `93: International Society for Optical Engineering (SPIE) conference,Los Angeles, CA (United States),13-23 Jan 1993; Other Information: PBD: [1993]
- Country of Publication:
- United States
- Language:
- English
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